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Development 2020 Jan 01; doi: 10.1242/dev.188094.
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Pinhead signaling regulates mesoderm heterogeneity via FGF receptor-dependent pathway.

Ossipova O , Itoh K , Radu A , Ezan J , Sokol SY .

Among the three embryonic germ layers, mesoderm plays a central role in the establishment of the vertebrate body plan. Mesoderm is specified by secreted signaling proteins from the FGF, Nodal, BMP and Wnt families. No new classes of extracellular mesoderm-inducing factors have been identified in more than two decades. Here we show that the pinhead (pnhd) gene encodes a secreted protein that is essential for the activation of a subset of mesodermal markers in the Xenopus embryo. RNA sequencing revealed that many transcriptional targets of Pnhd are shared with those of the FGF pathway. Pnhd activity was accompanied by Erk phosphorylation and required FGF and Nodal but not Wnt signaling. We propose that during gastrulation Pnhd acts in the marginal zone to contribute to mesoderm heterogeneity via an FGF receptor-dependent positive feedback mechanism.

PubMed ID: 34004873
Article link: Development

Species referenced: Xenopus laevis
Genes referenced: admp2 bmp7.1 cdx1 cdx2 cdx4 cer1 chrd.1 col17a1 dand5 dkk1 evx1 fgf2 fgf8 fgfr1 fgfr4 fkbp9 foxc1 foxc2 foxd4l1.1 frzb gjc2 grem1 hba1 hoxd1 kcnk6 mapk1 msgn1 myod1 nodal nodal3.1 nog nppb ntn3 pcdh8.2 pdgfra pnhd psmd6 pten sebox slc26a9 smad1 smad2 tbl1x tbx6 tbxt vangl2 wnt3a wnt8a zeb2 zg16
Antibodies: Ctnnb1 Ab9 FLAG Ab1 Smad1 Ab2
Morpholinos: pnhd MO4 pnhd MO5

Phenotypes: Xla Wt + Cco.FGFR4{del}-Hsa.IGHG3{del} (Fig.S7.C) [+]

Article Images: [+] show captions