Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Aging (Albany NY)
2021 Jan 19;132:1718-1728. doi: 10.18632/aging.202533.
Show Gene links
Show Anatomy links
Effects of alpha-cyclodextrin on water transport, cell hydration and longevity.
Szente L
,
Puskás I
,
Vellai T
,
Lohmann H
.
???displayArticle.abstract???
Among parent cyclodextrins (CDs), alpha-CD (a-CD) has been utilized in a number of nutraceutical products, and approved as a dietary fiber to affect glycemic response and reduce dietary fat absorption. To extend our current knowledge on the biology of this natural carbohydrate, here we investigated its potential effects on cellular water uptake and aging. Two independent in vivo biological test systems were used, a single cell Xenopus oocyte with expressed human aquaporin for cell hydration studies and the nematode Caenorhabditis elegans for testing life span in the treated animals. a-CD was found to enhance water uptake through aquaporins of oocytes. Furthermore, the compound promoted longevity in C. elegans. Together, these results raise a rational for assaying a-CD as a potent drug candidate in treating various age-related diseases.
Figure 1. Xenopus oocyte chamber with 6 oocytes (blue spheres) with different swelling and a calibration oocyte on the right of the photo.
Figure 2. Photographs of Xenopus oocyte chambers of 6 a-CD treated oocytes with different swelling due to water permeation into the cells and rupture of oocyte due to high volume expansion by water uptake. Photos were taken at increasing time points of treatment. The control un-swollen small oocyte is on the righthand site of each photo.
Figure 3. The normalized osmotic water permeability (PF values) of aquaporin expressed oocytes at 40 sec.
application of 0.05, 0.1 and 0.5 w/v% a-CD solutions vs. water control treatment. T-Test results against water control: ACD 0.05% (P=0.11 n.s.), ACD 0.1% (P<0.0001 ***) ACD 0.5%: (P=0.17 n.s.).
Figure 4. a-CD treatment can extend life span in C. elegans. (A) Kaplan-Meyer lifespan curve of control (untreated – blue curve) vs. a-CD-treated (0.05 w/v% - orange curve, 0.5 w/v% - green curve) animals. Animals treated with 0.05 w/v% a-CD live longer by 2 days on average than control. Both mean and maximum life spans become longer in response to treatment (see Supplementary Tables 1, 2). A higher concentration of a-CD (0.5 w/v%), however, does not lead to a longevity response. (B) a-CD at a working concentration of 0.1 w/v% slightly influences C. elegans life span. Kaplan-Meyer lifespan curves are shown. For statistics, see Supplementary Tables 1, 2.
Figure 5. a-CD extends C. elegans mean life span. Mean life span of nematodes treated with 0.05, 0.50 w/v% (A) and 0.10 w/v% (B) a-CD-containing water samples.
Agre,
Aquaporin water channels: molecular mechanisms for human diseases.
2003, Pubmed
Agre,
Aquaporin water channels: molecular mechanisms for human diseases.
2003,
Pubmed
Agre,
The aquaporin water channels.
2006,
Pubmed
Belikov,
Age-related diseases as vicious cycles.
2019,
Pubmed
Hovgaard,
Drug delivery studies in Caco-2 monolayers. IV. Absorption enhancer effects of cyclodextrins.
1995,
Pubmed
Kirkwood,
A systematic look at an old problem.
2008,
Pubmed
Leslie,
Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying.
1995,
Pubmed
Loftsson,
Self-association and cyclodextrin solubilization of drugs.
2002,
Pubmed
Luzardo,
Effect of trehalose and sucrose on the hydration and dipole potential of lipid bilayers.
2000,
Pubmed
Martínez-Ballesta,
Mutual Interactions between Aquaporins and Membrane Components.
2016,
Pubmed
Ohtani,
Differential effects of alpha-, beta- and gamma-cyclodextrins on human erythrocytes.
1989,
Pubmed
Sabadini,
Solubility of cyclomaltooligosaccharides (cyclodextrins) in H2O and D2O: a comparative study.
2006,
Pubmed
Seo,
Metabolic shift from glycogen to trehalose promotes lifespan and healthspan in Caenorhabditis elegans.
2018,
Pubmed
Szejtli,
Introduction and General Overview of Cyclodextrin Chemistry.
1998,
Pubmed
Tadeo,
Protein stabilization and the Hofmeister effect: the role of hydrophobic solvation.
2009,
Pubmed
Tóth,
Longevity pathways converge on autophagy genes to regulate life span in Caenorhabditis elegans.
2008,
Pubmed
Vellai,
The regulation of aging: does autophagy underlie longevity?
2009,
Pubmed
Vellai,
Autophagy genes and ageing.
2009,
Pubmed
Vellai,
Genetics: influence of TOR kinase on lifespan in C. elegans.
2003,
Pubmed
Vellai,
Regulation of protein turnover by longevity pathways.
2010,
Pubmed
Vollmer,
A study of the percutaneous absorption-enhancing effects of cyclodextrin derivatives in rats.
1994,
Pubmed
Weng,
Effects of Water on Structure and Dynamics of Trehalose Glasses at Low Water Contents and its Relationship to Preservation Outcomes.
2016,
Pubmed
