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Front Genet
2020 Mar 04;11:161. doi: 10.3389/fgene.2020.00161.
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Beta-Hydroxysteroid Dehydrogenase Genes in Orange-Spotted Grouper (Epinephelus coioides): Genome-Wide Identification and Expression Analysis During Sex Reversal.
Xiao L
,
Guo Y
,
Wang D
,
Zhao M
,
Hou X
,
Li S
,
Lin H
,
Zhang Y
.
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Beta-hydroxysteroid dehydrogenases (β-HSDs) are a group of steroidogenic enzymes that are involved in steroid biosynthesis and metabolism, and play a crucial role in mammalian physiology and development, including sex determination and differentiation. In the present study, a genome-wide analysis identified the numbers of β-hsd genes in orange-spotted grouper (Epinephelus coioides) (19), human (Homo sapiens) (22), mouse (Mus musculus) (24), chicken (Gallus gallus) (16), xenopus (Xenopus tropicalis) (24), coelacanth (Latimeria chalumnae) (17), spotted gar (Lepisosteus oculatus) (14), zebrafish (Danio rerio) (19), fugu (Takifugu rubripes) (19), tilapia (Oreochromis niloticus) (19), medaka (Oryzias latipes) (19), stickleback (Gasterosteus aculeatus) (17) and common carp (Cyprinus carpio) (27) samples. A comparative analysis revealed that the number of β-hsd genes in teleost fish was no greater than in tetrapods due to gene loss followed by a teleost-specific whole-genome duplication event. Based on transcriptome data from grouper brain and gonad samples during sex reversal, six β-hsd genes had relatively high expression levels in the brain, indicating that these genes may be required for neurogenesis or the maintenance of specific biological processes in the brain. In the gonad, two and eight β-hsd genes were up- and downregulated, respectively, indicating their important roles in sex reversal. Our results demonstrated that β-hsd genes may be involved in the sex reversal of grouper by regulating the synthesis and metabolism of sex steroid hormones.
FIGURE 1. Chromosome localization of 19 β-hsd genes from orange-spotted grouper.
FIGURE 2. Phylogenetic analysis, gene structure and conserved domains of β-hsd genes in orange-spotted grouper. (A) The phylogenetic tree of all β-Hsd in grouper was constructed using Neighbor-Joining method. (B) The exon/intron organization of β-hsd genes of grouper. The red boxes represent exons and black lines indicate introns. (C) The conserved protein domains in the β-Hsd were identified using CD-Search program. Each domain is indicated with a specific color. Yellow: NADB_Rossmann superfamily, green: PLN02864 superfamily, purple: SCP2 superfamily, blue: SDR superfamily.
FIGURE 3. Phylogenetic relationships of β-Hsd proteins from grouper and other five species. The phylogenetic tree was constructed using MEGA7 by Maximum Likelihood method with the amino acid sequences of β-Hsd from grouper (Epinephelus coioides, Ec), (Homo sapiens, Hs), mouse (Mus musculus, Mm), zebrafish (Danio rerio, Dr), tilapia (Oreochromis niloticus, On), and stickleback (Gasterosteus aculeatus, Ga).
FIGURE 4. Clustering of the expression profiles of 19 grouper β-hsd genes during sex reversal. Genes were clustered according to phylogenetic relationships in expression profiles. The RPKM values were transformed into Z scores. Z scores were plotted according to Z = (x - μ)/σ, where x is the log 2 transformed gene expression measurement and μ and σ are the mean and standard deviations of expression of the gene. Red and blue colors indicate high and low relative expression levels after homogenization, respectively. The RPKM value for each gene and each stage was provided in the figure.
FIGURE 5. Quantitative RT-PCR examination of 10 β-hsd genes expressions in the brain and gonad during sex reversal of grouper. Values represent the relative mRNA expression in relation to internal control (β-actin gene). Data were expressed as the mean ± SEM for four replicates. In each panel, different lowercase letters indicate significant differences (p < 0.05).
Alderman,
11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation.
2012, Pubmed
Alderman,
11β-Hydroxysteroid dehydrogenase type 2 in zebrafish brain: a functional role in hypothalamus-pituitary-interrenal axis regulation.
2012,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Baker,
Evolution of 17beta-hydroxysteroid dehydrogenases and their role in androgen, estrogen and retinoid action.
2001,
Pubmed
Belyaeva,
Comparative genomic and phylogenetic analysis of short-chain dehydrogenases/reductases with dual retinol/sterol substrate specificity.
2006,
Pubmed
Bhandari,
Gonadal restructuring and correlative steroid hormone profiles during natural sex change in protogynous honeycomb grouper (Epinephelus merra).
2003,
Pubmed
Chen,
Molecular cloning, characterization and expression profiles of three estrogen receptors in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides).
2011,
Pubmed
Cheng,
Genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in Nile tilapia, Oreochromis niloticus.
2015,
Pubmed
Cheng,
Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease.
2003,
Pubmed
Dai,
Molecular cloning and characterization of a novel human hydroxysteroid dehydrogenase-like 2 (HSDL2) cDNA from fetal brain.
2003,
Pubmed
Day,
Design and validation of specific inhibitors of 17beta-hydroxysteroid dehydrogenases for therapeutic application in breast and prostate cancer, and in endometriosis.
2008,
Pubmed
Fuller,
Mechanisms of ligand specificity of the mineralocorticoid receptor.
2012,
Pubmed
Godwin,
Neuroendocrinology of sexual plasticity in teleost fishes.
2010,
Pubmed
Hakkarainen,
Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.
2015,
Pubmed
He,
17β-Hydroxysteroid dehydrogenases and neurosteroid metabolism in the central nervous system.
2019,
Pubmed
Hoffmann,
Carbonyl reductases and pluripotent hydroxysteroid dehydrogenases of the short-chain dehydrogenase/reductase superfamily.
2007,
Pubmed
Hsu,
Zebrafish cyp11a1 and hsd3b genes: structure, expression and steroidogenic development during embryogenesis.
2009,
Pubmed
Hu,
GSDS 2.0: an upgraded gene feature visualization server.
2015,
Pubmed
Huang,
A novel human hydroxysteroid dehydrogenase like 1 gene (HSDL1) is highly expressed in reproductive tissues.
2001,
Pubmed
Jansson,
17beta-hydroxysteroid dehydrogenase 14 affects estradiol levels in breast cancer cells and is a prognostic marker in estrogen receptor-positive breast cancer.
2006,
Pubmed
Jeanmougin,
Multiple sequence alignment with Clustal X.
1998,
Pubmed
Järvensivu,
HSD17B1 expression enhances estrogen signaling stimulated by the low active estrone, evidenced by an estrogen responsive element-driven reporter gene in vivo.
2015,
Pubmed
Kasahara,
The medaka draft genome and insights into vertebrate genome evolution.
2007,
Pubmed
Kemiläinen,
The Hydroxysteroid (17β) Dehydrogenase Family Gene HSD17B12 Is Involved in the Prostaglandin Synthesis Pathway, the Ovarian Function, and Regulation of Fertility.
2016,
Pubmed
Kisiela,
Hydroxysteroid dehydrogenases (HSDs) in bacteria: a bioinformatic perspective.
2012,
Pubmed
Kobayashi,
Diversity and plasticity of sex determination and differentiation in fishes.
2013,
Pubmed
Kumar,
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.
2016,
Pubmed
Lin,
A challenge for medicinal chemistry by the 17β-hydroxysteroid dehydrogenase superfamily: an integrated biological function and inhibition study.
2013,
Pubmed
Liu,
Sexual plasticity: A fishy tale.
2017,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Lukacik,
Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity.
2007,
Pubmed
Lynch,
The evolutionary fate and consequences of duplicate genes.
2000,
Pubmed
Marchler-Bauer,
CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.
2017,
Pubmed
Meier,
Human and zebrafish hydroxysteroid dehydrogenase like 1 (HSDL1) proteins are inactive enzymes but conserved among species.
2009,
Pubmed
Mindnich,
Androgen metabolism via 17beta-hydroxysteroid dehydrogenase type 3 in mammalian and non-mammalian vertebrates: comparison of the human and the zebrafish enzyme.
2005,
Pubmed
Mindnich,
Zebrafish 17beta-hydroxysteroid dehydrogenases: an evolutionary perspective.
2009,
Pubmed
Mindnich,
Identification and characterization of 17 beta-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio.
2004,
Pubmed
Moummou,
The plant short-chain dehydrogenase (SDR) superfamily: genome-wide inventory and diversification patterns.
2012,
Pubmed
Murata,
Transient sex change in the immature Malabar grouper, Epinephelus malabaricus, androgen treatment.
2014,
Pubmed
Nakamoto,
Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes).
2012,
Pubmed
Oh,
Sex Reversal and Masculinization according to Growth in Longtooth Grouper Epinephelus bruneus.
2013,
Pubmed
Payne,
The multiple murine 3 beta-hydroxysteroid dehydrogenase isoforms: structure, function, and tissue- and developmentally specific expression.
1997,
Pubmed
Rotinen,
Type 10 17β-hydroxysteroid dehydrogenase expression is regulated by C/EBPβ in HepG2 cells.
2010,
Pubmed
Saloniemi,
The diversity of sex steroid action: novel functions of hydroxysteroid (17β) dehydrogenases as revealed by genetically modified mouse models.
2012,
Pubmed
Seckl,
11beta-hydroxysteroid dehydrogenases: changing glucocorticoid action.
2004,
Pubmed
Senthilkumaran,
Ovarian carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase shows distinct surge in messenger RNA expression during natural and gonadotropin-induced meiotic maturation in nile tilapia.
2002,
Pubmed
Sivik,
Expression patterns of 17β-hydroxysteroid dehydrogenase 14 in human tissues.
2012,
Pubmed
Sreenivasulu,
New evidences for the involvement of 20beta-hydroxysteroid dehydrogenase in final oocyte maturation of air-breathing catfish.
2009,
Pubmed
Tokarz,
Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-hydroxysteroid dehydrogenase type 2.
2012,
Pubmed
Tokarz,
Steroids in teleost fishes: A functional point of view.
2015,
Pubmed
Wei,
Genome-Wide Identification and Transcriptome-Based Expression Profiling of the Sox Gene Family in the Nile Tilapia (Oreochromis niloticus).
2016,
Pubmed
Xiao,
Comparative transcriptome analysis of diploid and triploid hybrid groupers (Epinephelus coioides♀ × E. lanceolatus♂) reveals the mechanism of abnormal gonadal development in triploid hybrids.
2019,
Pubmed
Xu,
Genome sequence and genetic diversity of the common carp, Cyprinus carpio.
2014,
Pubmed
Yang,
Roles of 17β-hydroxysteroid dehydrogenase type 10 in neurodegenerative disorders.
2014,
Pubmed
Yang,
3-Hydroxyacyl-CoA dehydrogenase and short chain 3-hydroxyacyl-CoA dehydrogenase in human health and disease.
2005,
Pubmed
Ye,
Environmental pollutants and hydroxysteroid dehydrogenases.
2014,
Pubmed
