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Dev Biol 2020 Mar 15;4592:138-148. doi: 10.1016/j.ydbio.2019.12.007.
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The secreted BMP antagonist ERFE is required for the development of a functional circulatory system in Xenopus.

Melchert J , Henningfeld KA , Richts S , Lingner T , Jonigk D , Pieler T .

The hormone Erythroferrone (ERFE) is a member of the C1q/TNF-related protein family that regulates iron homeostasis through the suppression of hamp. In a gain of function screen in Xenopus embryos, we identified ERFE as a potent secondary axis-inducing agent. Experiments in Xenopus embryos and ectodermal explants revealed that ERFE functions as a selective inhibitor of the BMP pathway and the conserved C1q domain is not required for this activity. Inhibition occurs at the extracelluar level, through the interaction of ERFE with the BMP ligand. During early Xenopus embryogenesis, erfe is first expressed in the ventral blood islands where initial erythropoiesis occurs and later in circulating blood cells. ERFE knockdown does not alter the expression of etv.2, aplnr and flt1 in tailbud stage embryos indicating endothelial cell specification is independent of ERFE. However, in tadpole embryos, defects of the vascular network and primitive blood circulation are observed as well as edema formation. RNAseq analysis of ERFE morphant embryos also revealed the inhibition of gja4 indicating disruption of dorsal aorta formation.

PubMed ID: 31846624
Article link: Dev Biol

Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: aplnr babam2 bmp2 bmp4 cfd erfe etv2 fes fgf4 flt1 gja4 h4c4 hba3 myc ncam1 nodal3.1 nog odc1 ppil1 rbpj tbxt ventx1 ventx1.2 wnt8a
GO keywords: circulatory system process [+]
Antibodies: HA Ab3 Myc Ab18
Morpholinos: erfe MO1 erfe MO2

GEO Series: GSE126058: Xenbase,  NCBI

Article Images: [+] show captions