Umair Z et al. (2018), Ventx1.1 as a Direct Repressor of Early Neural ...

XB-ART-55575

Mol Cells 2018 Dec 31;4112:1061-1071. doi: 10.14348/molcells.2018.0341.

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Ventx1.1 as a Direct Repressor of Early Neural Gene zic3 in Xenopus laevis.

Umair Z , Kumar S , Kim DH , Rafiq K , Kumar V , Kim S , Park JB , Lee JY , Lee U , Kim J .

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From Xenopus embryo studies, the BMP4/Smad1-targeted gene circuit is a key signaling pathway for specifying the cell fate between the ectoderm and neuro-ectoderm as well as the ventral and dorsal mesoderm. In this context, several BMP4/Smad1 target transcriptional factors have been identified as repressors of the neuro-ectoderm. However, none of these direct target transcription factors in this pathway, including GATA1b, Msx1 and Ventx1.1 have yet been proven as direct repressors of early neuro-ectodermal gene expression. In order to demonstrate that Ventx1.1 is a direct repressor of neuro-ectoderm genes, a genome-wide Xenopus ChIP-Seq of Ventx1.1 was performed. In this study, we demonstrated that Ventx1.1 bound to the Ventx1.1 response cis-acting element 1 and 2 (VRE1 and VRE2) on the promoter for zic3, which is a key early neuro-ectoderm gene, and this Ventx1.1 binding led to repression of zic3 transcription. Site-directed mutagenesis of VRE1 and VRE2 within zic3 promoter completely abolished the repression caused by Ventx1.1. In addition, we found both the positive and negative regulation of zic3 promoter activity by FoxD5b and Xcad2, respectively, and that these occur through the VREs and via modulation of Ventx1.1 levels. Taken together, the results demonstrate that the BMP4/Smad1 target gene, Ventx1.1, is a direct repressor of neuro-ectodermal gene zic3 during early Xenopus embryogenesis.

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Species referenced: Xenopus laevis
Genes referenced: cdx2 foxd4l1.1 krt12.4 mrc1 msx1 ncam1 neurod1 ventx1 ventx1.2 zic3

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	Fig. 1. Ventx1.1 represses early and late neural genes in Xenopus whole embryo and animal cap explantsVentx1.1 (500 pg/embryo) mRNA was co-injected with and without FoxD5b-En mRNA (280 pg/embryos) at the one-cell stage, followed by dissection of the animal cap explants (AC) at stage 8 and harvesting at stage 11 and 24 respectively. The relative gene expressions were analyzed by RT-PCR: (A and C) Ventx1.1 reduced the FoxD5b-induced expression of zic3 and foxD5b in both animal cap explants (A) and whole embryos (WE) (B) at stage 11. (B and D) Ventx1.1 reduced the FoxD5b-En-induced expression of ncam, Xngnr (pan-neural markers) and NeuroD (neuronal differentiation marker) while induced the expression of xK81 (keratin marker) at stage 24. EF1α loading control, non-injected animal caps (negative control), whole embryo (WE) (positive control), control reaction without reverse transcriptase (−RT).
	Fig. 2. Genome-wide Xenopus ChIP-Seq of Ventx1.1 identifies two Ventx1.1 response cisacting elements (VRE1 and VRE2) on the zic3 promoter(A) Putative Ventx1.1 response consensus binding elements (VREs) are presented within the upstream promoter region of zic3 gene. (B) ChIP-Seq coverage plot of 3Flag-Ventx1.1 on the zic3 promoter. (C) Putative VRE sequences and their locations within the zic3 promoter. (D) This plot is showing the similarity in structure between Chr8S and Chr8L of zic3 promoter with the VRE1 and VRE2 locations marked. (E) In the ChIP assay, 3Flag-Ventx1.1 construct (500 pg/embryos) was injected at the one-cell stage and the embryos were harvested at stage 11 in 30% MMR solution. Anti-Flag antibody was used to immune-precipitate the endogenous zic3 promoter region. VRE1 and VRE2 were measured by PCR with specific zic3 promoter primers. (F) Consensus binding motifs of VRE1 and VRE2 are fully matched in both Chr8S and Chr8L of the zic3 promoter regions.
	Fig. 3. Site-directed mutagenesis of VRE1 and VRE2 within the zic3 promoter-reporter construct abolishes the repressional activity of Ventx1.1. on the reporter(A) Schematic representation of serially-deleted zic3 promoter constructs. (A–I) Different serially-deleted zic3 (40 pg/embryos) promoter constructs were co-injected with and without Ventx1.1 (500 pg/embryos) at the one-cell stage and grown until stage 11 to measure the relative promoter activity. The data are shown as mean ± S.E. of the values from at least three independent experiments. Differences were considered significant at P < 0.05.
	Fig. 4. VRE1 mutation partially abolishes negative and positive effects of Xcad2 and FoxD5b-En, respectivelyDifferent serially-deleted zic3(40 pg/embryos) promoter constructs were co-injected with and without FoxD5b-En (280 pg/embryos) at the one-cell stage and grown until stage 11 to measure the relative promoter activity. (A) FoxD5b-En increased the relative promoter activity of zic3(−1805) promoter region compared to zic3(−1805) alone. (B) zic3(−517) and zic3(−517)mVRE1 promoter constructs were co-injected with and without FoxD5b-En in separate groups. The FoxD5b-mediated induction in the relative promoter activity of zic3(−517) was abolished in zic3(−517)mVRE1. (C) zic3(−517) and zic3(−517)mVRE1 promoter constructs were co-injected with and without Xcad2 in separate groups. The Xcad2-mediated reduction in the relative promoter activity of zic3(−517) was abolished in zic3(−517)mVRE1 reporter construct. The data are shown as mean ± S.E. of the values from at least three independent experiments. Differences were considered significant at P < 0.05.
	Fig. 5. Schematic diagram depicting Ventx1.1 as a key protein in the neural inhibitory circuit of BMP4/Smad1/Xcad2/Ventx1.1 and the reciprocal inhibitory circuit of FoxD5b/Ventx1.1Ventx1.1 seems play an essential role in the BMP4/Smad1/Xcad2/Ventx1.1-mediated neural inhibition in Xenopus embryos during gastrula.

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Ault, A novel homeobox gene PV.1 mediates induction of ventral mesoderm in Xenopus embryos. 1996, Pubmed, Xenbase