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Development 2018 Nov 21;14522:. doi: 10.1242/dev.164269.
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The Ric-8A/Gα13/FAK signalling cascade controls focal adhesion formation during neural crest cell migration in Xenopus.

Toro-Tapia G , Villaseca S , Beyer A , Roycroft A , Marcellini S , Mayor R , Torrejón M .

Ric-8A is a pleiotropic guanine nucleotide exchange factor involved in the activation of various heterotrimeric G-protein pathways during adulthood and early development. Here, we sought to determine the downstream effectors of Ric-8A during the migration of the vertebrate cranial neural crest (NC) cells. We show that the Gα13 knockdown phenocopies the Ric-8A morphant condition, causing actin cytoskeleton alteration, protrusion instability, and a strong reduction in the number and dynamics of focal adhesions. In addition, the overexpression of Gα13 is sufficient to rescue Ric-8A-depleted cells. Ric-8A and Gα13 physically interact and colocalize in protrusions of the cells leading edge. The focal adhesion kinase FAK colocalizes and interacts with the endogenous Gα13, and a constitutively active form of Src efficiently rescues the Gα13 morphant phenotype in NC cells. We propose that Ric-8A-mediated Gα13 signalling is required for proper cranial NC cell migration by regulating focal adhesion dynamics and protrusion formation.

PubMed ID: 30297374
PMC ID: PMC6262797
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: cdh1 cdh2 fn1 gna13 itgb1 itk myc ptk2 pxn ric8a slc5a5 snai2 twist1
GO keywords: neural crest cell migration [+]
Antibodies: Cdh1 Ab1 Cdh2 Ab3 Itgb1 Ab1 Myc Ab5 Pxn Ab2 gna13 Ab1
Morpholinos: gna13 MO2 ric8a MO1

Article Images: [+] show captions
References [+] :
Alfandari, Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin. 2003, Pubmed, Xenbase