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iScience 2018 Apr 27;2:76-85. doi: 10.1016/j.isci.2018.03.011.
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An Early Function of Polycystin-2 for Left-Right Organizer Induction in Xenopus.

Vick P , Kreis J , Schneider I , Tingler M , Getwan M , Thumberger T , Beyer T , Schweickert A , Blum M .

Nodal signaling controls asymmetric organ placement during vertebrate embryogenesis. Nodal is induced by a leftward fluid flow at the ciliated left-right organizer (LRO). The mechanism of flow sensing, however, has remained elusive. pkd2 encodes the calcium channel Polycystin-2, which is required for kidney development and laterality, and may act in flow perception. Here, we have studied the role of Polycystin-2 in Xenopus and show that pkd2 is indispensable for left-right (LR) asymmetry. Knockdown of pkd2 prevented left-asymmetric nodal cascade induction in the lateral plate mesoderm. Defects were due to failure of LRO specification, morphogenesis, and, consequently, absence of leftward flow. Polycystin-2 synergizes with the unconventional nodal-type signaling molecule Xnr3 to induce the LRO precursor tissue before gastrulation, upstream of symmetry breakage. Our data uncover an unknown function of pkd2 in LR axis formation, which we propose represents an ancient role of Polycystin-2 during LRO induction in lower vertebrates.

PubMed ID: 30428378
PMC ID: PMC6136938
Article link: iScience

Species referenced: Xenopus
Genes referenced: adm dand5 dnah9 fgfr1 foxj1 foxj1.2 lefty nodal nodal1 nodal3.1 pitx2 pkd2 rho tbxt tekt2 tnfsf10 wnt8a
GO keywords: nodal signaling pathway
Morpholinos: pkd2 MO1

Article Images: [+] show captions
References [+] :
Beyer, Serotonin signaling is required for Wnt-dependent GRP specification and leftward flow in Xenopus. 2012, Pubmed, Xenbase