XB-ART-55241
Methods Mol Biol
2018 Jan 01;1865:55-65. doi: 10.1007/978-1-4939-8784-9_4.
Show Gene links
Show Anatomy links
Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology.
???displayArticle.abstract???
Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward transcription activator-like effector nucleases (TALENs), adapted from plant pathogenic Xanthomonas species. Although functional genomics are more recently dominated by the even faster and more convenient CRISPR/Cas9 technology, the use of TALENs may still be preferred in a number of cases. We have successfully implemented this technology in Xenopus and in this chapter we describe our working protocol for targeted genome editing in X. tropicalis using TALENs.
???displayArticle.pubmedLink??? 30151758
???displayArticle.link??? Methods Mol Biol