Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Mech Dev 2018 Dec 01;154:219-239. doi: 10.1016/j.mod.2018.07.010.
Show Gene links Show Anatomy links

Gli2 is required for the induction and migration of Xenopus laevis neural crest.

Cerrizuela S , Vega-López GA , Palacio MB , Tríbulo C , Aybar MJ .

The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial bones and cartilages, peripheral glial cells, and melanocyte cells, among others. In this work we analyzed the role of the Hedgehog signaling pathway effector gli2 in Xenopus NC. We provide evidence that the gli2 gene is expressed in the prospective, premigratory and migratory NC. The use of a specific morpholino against gli2 and the pharmacological specific inhibitor GANT61 in different experimental approaches allowed us to determine that gli2 is required for the induction and specification of NC cells as a transcriptional activator. Moreover, gli2 also acts by reducing apoptosis in the NC without affecting its cell proliferation status. We also demonstrated that gli2 is required cell-autonomously for NC migration, and for the formation of NC derivatives such as the craniofacial cartilages, melanocytes and the cranial ganglia. Altogether, our results showed that gli2 is a key transcriptional activator to accomplish the proper specification and development of Xenopus NC cells.

PubMed ID: 30086335
Article link: Mech Dev

Species referenced: Xenopus laevis
Genes referenced: cad cdh1 cdh2 dlx5 ednra foxd3 gli2 gli3 hes4 ihh kif11 krt12.4 msx1 myc npb pax3 ptch1 shh six1 slc12a3 snai1 snai2 sox10 sox2 sox9 tbx2 tuba1cl.3 twist1
GO keywords: neural crest cell migration [+]
Antibodies: Ptch1 Ab2 Tuba1a Ab1
Morpholinos: ednra MO1 gli2 MO2 gli3 MO1 ihh MO1 kif11 MO1

Article Images: [+] show captions