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Dev Biol 2018 Oct 15;4422:262-275. doi: 10.1016/j.ydbio.2018.07.025.
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Xenopus SOX5 enhances myogenic transcription indirectly through transrepression.

Della Gaspera B , Chesneau A , Weill L , Charbonnier F , Chanoine C .

In anamniotes, somite compartimentalization in the lateral somitic domain leads simultaneously to myotome and dermomyotome formation. In the myotome, Xenopus Sox5 is co-expressed with Myod1 in the course of myogenic differentiation. Here, we studied the function of Sox5 using a Myod1-induced myogenic transcription assay in pluripotent cells of animal caps. We found that Sox5 enhances myogenic transcription of muscle markers Des, Actc1, Ckm and MyhE3. The use of chimeric transactivating or transrepressive Sox5 proteins indicates that Sox5 acts as a transrepressor and indirectly stimulates myogenic transcription except for the slow muscle-specific genes Myh7L, Myh7S, Myl2 and Tnnc1. We showed that this role is shared by Sox6, which is structurally similar to Sox5, both belonging to the SoxD subfamily of transcription factors. Moreover, Sox5 can antagonize the inhibitory function of Meox2 on myogenic differentiation. Meox2 which is a dermomyotome marker, represses myogenic transcription in Myod-induced myogenic transcription assay and in Nodal5-induced mesoderm from animal cap assay. The inhibitory function of Meox2 and the pro-myogenic function of Sox5 were confirmed during Xenopus normal development by the use of translation-blocking oligomorpholinos and dexamethasone inducible chimeric Sox5 and Meox2 proteins. We have therefore identified a new function for SoxD proteins in muscle cells, which can indirectly enhance myogenic transcription through transrepression, in addition to the previously identified function as a direct repressor of slow muscle-specific genes.

PubMed ID: 30071218
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: actc1 ash2l bhlhe41 cbx5 ccnd1 cdknx ckm des.1 des.2 ezh2 foxc1 foxc2 h1-0 h2az1 h3-3b h3-5 hdac1 hdac3 hdac4 hdac9 hey1 id2 id3 id4 macroh2a1 macroh2a2 map2k1 mef2a mef2c mef2d meox2 myf5 myf6 myh2 myh4 myh7 myl11 myl2 myod1 myog nfat5 nfatc1 nfatc2 nfatc3 nfia nfib nfix nodal5 nodal5.5 pax3 six1 six4 smarca2 sox15 sox5 sox6 sox8 sox9 tcf15 tcf3 tcf4 tnnc1 tnnc2 tp53 ventx1.2 ventx2 yy1 zbtb18
Antibodies: FLAG Ab1
Morpholinos: meox2 MO1 meox2 MO2 sox5 MO4

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