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Dev Dyn 2018 Sep 01;2479:1070-1082. doi: 10.1002/dvdy.24659.
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Nosip functions during vertebrate eye and cranial cartilage development.

Flach H , Krieg J , Hoffmeister M , Dietmann P , Reusch A , Wischmann L , Kernl B , Riegger R , Oess S , Kühl SJ .

BACKGROUND: The nitric oxide synthase interacting protein (Nosip) has been associated with diverse human diseases including psychological disorders. In line, early neurogenesis of mouse and Xenopus is impaired upon Nosip deficiency. Nosip knockout mice show craniofacial defects and the down-regulation of Nosip in the mouse and Xenopus leads to microcephaly. Until now, the exact underlying molecular mechanisms of these malformations were still unknown. RESULTS: Here, we show that nosip is expressed in the developing ocular system as well as the anterior neural crest cells of Xenopus laevis. Furthermore, Nosip inhibition causes severe defects in eye formation in the mouse and Xenopus. Retinal lamination as well as dorso-ventral patterning of the retina were affected in Nosip-depleted Xenopus embryos. Marker gene analysis using rax, pax6 and otx2 reveals an interference with the eye field induction and differentiation. A closer look on Nosip-deficient Xenopus embryos furthermore reveals disrupted cranial cartilage structures and an inhibition of anterior neural crest cell induction and migration shown by twist, snai2, and egr2. Moreover, foxc1 as downstream factor of retinoic acid signalling is affected upon Nosip deficiency. CONCLUSIONS: Nosip is a crucial factor for the development of anterior neural tissue such the eyes and neural crest cells. Developmental Dynamics 247:1070-1082, 2018. © 2018 Wiley Periodicals, Inc.

PubMed ID: 30055071
Article link: Dev Dyn

Species referenced: Xenopus laevis
Genes referenced: egr2 foxc1 nosip otx2 pax6 pou4f1 prox1 rax rho rpe snai2 sox3 tbx3 twist1 vax2 vsx1
Morpholinos: nosip MO1

Disease Ontology terms: microphthalmia [+]

Article Images: [+] show captions