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Cell Cycle
2018 Jan 01;176:739-748. doi: 10.1080/15384101.2018.1442630.
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RCC1 regulates inner centromeric composition in a Ran-independent fashion.
Zhang MS
,
Furuta M
,
Arnaoutov A
,
Dasso M
.
Abstract
RCC1 associates to chromatin dynamically within mitosis and catalyzes Ran-GTP production. Exogenous RCC1 disrupts kinetochore structure in Xenopus egg extracts (XEEs), but the molecular basis of this disruption remains unknown. We have investigated this question, utilizing replicated chromosomes that possess paired sister kinetochores. We find that exogenous RCC1 evicts a specific subset of inner KT proteins including Shugoshin-1 (Sgo1) and the chromosome passenger complex (CPC). We generated RCC1 mutants that separate its enzymatic activity and chromatin binding. Strikingly, Sgo1 and CPC eviction depended only on RCC1's chromatin affinity but not its capacity to produce Ran-GTP. RCC1 similarly released Sgo1 and CPC from synthetic kinetochores assembled on CENP-A nucleosome arrays. Together, our findings indicate RCC1 regulates kinetochores at the metaphase-anaphase transition through Ran-GTP-independent displacement of Sgo1 and CPC.
Arnaoutov,
The Ran GTPase regulates kinetochore function.
2003, Pubmed,
Xenbase
Arnaoutov,
The Ran GTPase regulates kinetochore function.
2003,
Pubmed
,
Xenbase
Azuma,
Model of the ran-RCC1 interaction using biochemical and docking experiments.
1999,
Pubmed
Bischoff,
A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.
1990,
Pubmed
Boiarchuk,
[Assembly of correct kinetochore architecture in Xenopus laevis egg extract requires transition of sperm DNA through interphase].
2007,
Pubmed
,
Xenbase
Boyarchuk,
Bub1 is essential for assembly of the functional inner centromere.
2007,
Pubmed
,
Xenbase
Carmena,
The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis.
2013,
Pubmed
Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed
Dasso,
A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation.
1995,
Pubmed
,
Xenbase
Earnshaw,
The use of autoantibodies in the study of nuclear and chromosomal organization.
1992,
Pubmed
Fukagawa,
The centromere: chromatin foundation for the kinetochore machinery.
2014,
Pubmed
Furuta,
Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.
2016,
Pubmed
Guse,
A cell-free system for functional centromere and kinetochore assembly.
2013,
Pubmed
,
Xenbase
Gutiérrez-Caballero,
Shugoshins: from protectors of cohesion to versatile adaptors at the centromere.
2012,
Pubmed
Hutchins,
Phosphorylation regulates the dynamic interaction of RCC1 with chromosomes during mitosis.
2004,
Pubmed
,
Xenbase
Kawashima,
Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin.
2010,
Pubmed
Makde,
Structure of RCC1 chromatin factor bound to the nucleosome core particle.
2010,
Pubmed
,
Xenbase
Milks,
Dissection of CENP-C-directed centromere and kinetochore assembly.
2010,
Pubmed
,
Xenbase
Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Nemergut,
Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B.
2001,
Pubmed
,
Xenbase
Renault,
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).
2001,
Pubmed
Rodriguez-Collazo,
Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.
2009,
Pubmed
Stewart,
The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2).
1999,
Pubmed
,
Xenbase
Watanabe,
Temporal and spatial regulation of targeting aurora B to the inner centromere.
2011,
Pubmed
Zhang,
RanBP1 governs spindle assembly by defining mitotic Ran-GTP production.
2015,
Pubmed
,
Xenbase
