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J Biol Chem
2017 Nov 17;29246:18924-18936. doi: 10.1074/jbc.M117.811109.
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ZNF143 protein is an important regulator of the myeloid transcription factor C/EBPα.
Gonzalez D
,
Luyten A
,
Bartholdy B
,
Zhou Q
,
Kardosova M
,
Ebralidze A
,
Swanson KD
,
Radomska HS
,
Zhang P
,
Kobayashi SS
,
Welner RS
,
Levantini E
,
Steidl U
,
Chong G
,
Collombet S
,
Choi MH
,
Friedman AD
,
Scott LM
,
Alberich-Jorda M
,
Tenen DG
.
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The transcription factor C/EBPα is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, ∼100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBPα expression. This sequence is present in the genes encoding C/EBPα in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBPα expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBPα in myeloid cells.
Agrawal,
The C/EBPdelta tumor suppressor is silenced by hypermethylation in acute myeloid leukemia.
2007, Pubmed
Agrawal,
The C/EBPdelta tumor suppressor is silenced by hypermethylation in acute myeloid leukemia.
2007,
Pubmed
Alberich-Jordà,
C/EBPγ deregulation results in differentiation arrest in acute myeloid leukemia.
2012,
Pubmed
Avellino,
An autonomous CEBPA enhancer specific for myeloid-lineage priming and neutrophilic differentiation.
2016,
Pubmed
Badis,
Diversity and complexity in DNA recognition by transcription factors.
2009,
Pubmed
Bailey,
ZNF143 provides sequence specificity to secure chromatin interactions at gene promoters.
2015,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Chen,
Zfp143 regulates Nanog through modulation of Oct4 binding.
2008,
Pubmed
Chen,
The Sp1 transcription factor binds the CD11b promoter specifically in myeloid cells in vivo and is essential for myeloid-specific promoter activity.
1993,
Pubmed
Chia,
A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.
2010,
Pubmed
Dejosez,
Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells.
2010,
Pubmed
Ford,
Regulation of the myeloperoxidase enhancer binding proteins Pu1, C-EBP alpha, -beta, and -delta during granulocyte-lineage specification.
1996,
Pubmed
Frank,
Binding of c-Myc to chromatin mediates mitogen-induced acetylation of histone H4 and gene activation.
2001,
Pubmed
Gombart,
Neutrophil-specific granule deficiency: homozygous recessive inheritance of a frameshift mutation in the gene encoding transcription factor CCAAT/enhancer binding protein--epsilon.
2001,
Pubmed
Grossman,
ZNF143 mediates basal and tissue-specific expression of human transaldolase.
2004,
Pubmed
Guo,
In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis.
2016,
Pubmed
Guo,
Runx1 deletion or dominant inhibition reduces Cebpa transcription via conserved promoter and distal enhancer sites to favor monopoiesis over granulopoiesis.
2012,
Pubmed
Halbig,
The transcriptional activator ZNF143 is essential for normal development in zebrafish.
2012,
Pubmed
Heidari,
Genome-wide map of regulatory interactions in the human genome.
2014,
Pubmed
Holt,
Downregulation of repressive CUP/AP-2 isoforms during adipocyte differentiation.
2001,
Pubmed
Iwama,
Dimeric RFX proteins contribute to the activity and lineage specificity of the interleukin-5 receptor alpha promoter through activation and repression domains.
1999,
Pubmed
Izumi,
Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell-cycle-associated genes.
2010,
Pubmed
Khanna-Gupta,
Sp1 and C/EBP are necessary to activate the lactoferrin gene promoter during myeloid differentiation.
2000,
Pubmed
Klinakis,
A novel tumour-suppressor function for the Notch pathway in myeloid leukaemia.
2011,
Pubmed
Koschmieder,
Dysregulation of the C/EBPalpha differentiation pathway in human cancer.
2009,
Pubmed
Landschulz,
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
1988,
Pubmed
Lekstrom-Himes,
Biological role of the CCAAT/enhancer-binding protein family of transcription factors.
1998,
Pubmed
López-Rodríguez,
Identification of Sp1-binding sites in the CD11c (p150,95 alpha) and CD11a (LFA-1 alpha) integrin subunit promoters and their involvement in the tissue-specific expression of CD11c.
1995,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Mead,
Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins.
1998,
Pubmed
,
Xenbase
Myslinski,
ZNF76 and ZNF143 are two human homologs of the transcriptional activator Staf.
1998,
Pubmed
,
Xenbase
Nerlov,
The C/EBP family of transcription factors: a paradigm for interaction between gene expression and proliferation control.
2007,
Pubmed
Ngondo-Mbongo,
Modulation of gene expression via overlapping binding sites exerted by ZNF143, Notch1 and THAP11.
2013,
Pubmed
Niebuhr,
Investigation of C/EBPalpha function in human (versus murine) myelopoiesis provides novel insight into the impact of CEBPA mutations in acute myelogenous leukemia (AML).
2009,
Pubmed
Nordeen,
Luciferase reporter gene vectors for analysis of promoters and enhancers.
1988,
Pubmed
Ogawa,
Differentiation and proliferation of hematopoietic stem cells.
1993,
Pubmed
Pabst,
Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia.
2001,
Pubmed
Pahl,
Optimization of transient transfection into human myeloid cell lines using a luciferase reporter gene.
1991,
Pubmed
Parker,
Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143.
2014,
Pubmed
Radomska,
CCAAT/enhancer binding protein alpha is a regulatory switch sufficient for induction of granulocytic development from bipotential myeloid progenitors.
1998,
Pubmed
Ranganathan,
Notch signalling in solid tumours: a little bit of everything but not all the time.
2011,
Pubmed
Rhee,
Comprehensive genome-wide protein-DNA interactions detected at single-nucleotide resolution.
2011,
Pubmed
Rosenbauer,
Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU.1.
2004,
Pubmed
Schaub,
Maximization of selenocysteine tRNA and U6 small nuclear RNA transcriptional activation achieved by flexible utilization of a Staf zinc finger.
1999,
Pubmed
,
Xenbase
Schaub,
Structural organization of Staf-DNA complexes.
2000,
Pubmed
,
Xenbase
Schuster,
Staf, a novel zinc finger protein that activates the RNA polymerase III promoter of the selenocysteine tRNA gene.
1995,
Pubmed
,
Xenbase
Schödel,
High-resolution genome-wide mapping of HIF-binding sites by ChIP-seq.
2011,
Pubmed
Smith,
PU.1 (Spi-1) and C/EBP alpha regulate the granulocyte colony-stimulating factor receptor promoter in myeloid cells.
1996,
Pubmed
Tenen,
Transcription factors, normal myeloid development, and leukemia.
1997,
Pubmed
Timchenko,
Autoregulation of the human C/EBP alpha gene by stimulation of upstream stimulatory factor binding.
1995,
Pubmed
Todeschini,
Transcription factors: specific DNA binding and specific gene regulation.
2014,
Pubmed
Wakasugi,
ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes.
2007,
Pubmed
,
Xenbase
Wang,
Genome-wide analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells.
2011,
Pubmed
Williams,
A rapid method for determining the molecular weight of a protein bound to nucleic acid in a mobility shift assay.
1992,
Pubmed
Ye,
C/EBPa controls acquisition and maintenance of adult haematopoietic stem cell quiescence.
2013,
Pubmed
Yin,
Notch signaling inhibits the growth of the human chronic myeloid leukemia cell line K562.
2009,
Pubmed
Yuan,
CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.
2007,
Pubmed
Zaret,
Selection and analysis of galactose metabolic pathway variants of a mouse liver cell line.
1990,
Pubmed
Zhang,
Sp1 is a critical factor for the monocytic specific expression of human CD14.
1994,
Pubmed
Zhang,
Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha.
2004,
Pubmed
Zhang,
Absence of granulocyte colony-stimulating factor signaling and neutrophil development in CCAAT enhancer binding protein alpha-deficient mice.
1997,
Pubmed
Zheng,
ZNF76, a novel transcriptional repressor targeting TATA-binding protein, is modulated by sumoylation.
2004,
Pubmed
