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J Cell Sci 2017 Jan 15;1302:420-428. doi: 10.1242/jcs.194704.
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RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells.

Sedzinski J , Hannezo E , Tu F , Biro M , Wallingford JB .

Homeostatic replacement of epithelial cells from basal precursors is a multistep process involving progenitor cell specification, radial intercalation and, finally, apical surface emergence. Recent data demonstrate that actin-based pushing under the control of the formin protein Fmn1 drives apical emergence in nascent multiciliated epithelial cells (MCCs), but little else is known about this actin network or the control of Fmn1. Here, we explore the role of the small GTPase RhoA in MCC apical emergence. Disruption of RhoA function reduced the rate of apical surface expansion and decreased the final size of the apical domain. Analysis of cell shapes suggests that RhoA alters the balance of forces exerted on the MCC apical surface. Finally, quantitative time-lapse imaging and fluorescence recovery after photobleaching studies argue that RhoA works in concert with Fmn1 to control assembly of the specialized apical actin network in MCCs. These data provide new molecular insights into epithelial apical surface assembly and could also shed light on mechanisms of apical lumen formation.

PubMed ID: 28089989
PMC ID: PMC5278671
Article link: J Cell Sci
Grant support: [+]

Species referenced: Xenopus
Genes referenced: fmn1 mcc mtor rhoa tuba4b utrn
Morpholinos: fmn1 MO2

Article Images: [+] show captions
References [+] :
Benink, Concentric zones of active RhoA and Cdc42 around single cell wounds. 2005, Pubmed, Xenbase