XB-ART-52510Biomed Opt Express 2016 Mar 30;74:1590-603. doi: 10.1364/BOE.7.001590.
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Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale fluid flows.
We present a new OCT method for flow speed quantification and directional velocimetry: particle streak velocimetry-OCT (PSV-OCT). PSV-OCT generates two-dimensional, 2.5-vector component (vx ,|vy |,vz ) maps of microscale flow velocity fields. Knowledge of 2.5-vector components also enables the estimation of total flow speed. The enabling insight behind PSV-OCT is that tracer particles in sparsely-seeded fluid flow trace out streaks in (x,z,t)-space. The streak orientations in x-t and z-t yield vx and vz , respectively. The in-plane (x-z plane) residence time yields the out-of-plane speed |vy |. Vector component values are generated by fitting streaks to a model of image formation that incorporates equations of motion in 3D space. We demonstrate cross-sectional estimation of (vx ,|vy |,vz ) in two important animal models in ciliary biology: Xenopus embryos (tadpoles) and mouse trachea.
PubMed ID: 27375926
PMC ID: PMC4929663
Article link: Biomed Opt Express
References [+] :
Ahn, Quantification of a three-dimensional velocity vector using spectral-domain Doppler optical coherence tomography. 2007, Pubmed