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Development 2016 Nov 01;14321:3944-3955. doi: 10.1242/dev.138917.
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Members of the Rusc protein family interact with Sufu and inhibit vertebrate Hedgehog signaling.

Jin Z , Schwend T , Fu J , Bao Z , Liang J , Zhao H , Mei W , Yang J .

Hedgehog (Hh) signaling is fundamentally important for development and adult tissue homeostasis. It is well established that in vertebrates Sufu directly binds and inhibits Gli proteins, the downstream mediators of Hh signaling. However, it is unclear how the inhibitory function of Sufu towards Gli is regulated. Here we report that the Rusc family of proteins, the biological functions of which are poorly understood, form a heterotrimeric complex with Sufu and Gli. Upon Hh signaling, Rusc is displaced from this complex, followed by dissociation of Gli from Sufu. In mammalian fibroblast cells, knockdown of Rusc2 potentiates Hh signaling by accelerating signaling-induced dissociation of the Sufu-Gli protein complexes. In Xenopus embryos, knockdown of Rusc1 or overexpression of a dominant-negative Rusc enhances Hh signaling during eye development, leading to severe eye defects. Our study thus uncovers a novel regulatory mechanism controlling the response of cells to Hh signaling in vertebrates.

PubMed ID: 27633991
PMC ID: PMC5117142
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: chrd.1 gli1 gli2 hhip myc odc1 pax6 ptch1 ptch2 rax rusc1 rusc2 shh six3 sufu
Morpholinos: gli1 MO1 rusc1 MO1 rusc1 MO2 rusc2 MO1 rusc2 MO2

Article Images: [+] show captions
References [+] :
Aavikko, Loss of SUFU function in familial multiple meningioma. 2012, Pubmed