Bosse JL , El-Hodiri HM .

R01 EY015480 NEI NIH HHS

	Fig. 1. insm1 is expressed in retinal progenitor cells (RPCs). A. Schematic representation of the mature Xenopus tadpole retina. B – J. In situ hybridization performed using sectioned Xenopus laevis embryos. insm1 is expressed in retinal progenitor cells (RPCs) and undifferentiated neuroepithelium at stage 38. At stages 41 (C, H) and stage 45 (D), insm1 expression was detected in RPCs located in zones 2 and 3 of the ciliary marginal zone (CMZ) but not in the retinal stem cells of zone 1. Compare the expression of insm1 at stage 41 (C, H) to expression of notch1, a zone 2 marker, at stage 41 (F, I). Both are absent in the most distal zone 1 of the CMZ. Also compare the proximal expression of insm1 (C, H) to zone 3 and 4 marker, neuroD at stage 41 (G, J). The CMZ is indicated by black brackets. Scale bars (1 μM) for (B – G) and (H – J) are indicated in (A) and (F), respectively. Abbreviations: GL – ganglion cell layer, INL – inner nuclear layer, PL – photoreceptor layer.
	Fig. 2. insm1 expression in the developing brain. A – C. Diagrammatic representation of selected brain regions as viewed in dorsally (A), ventrally (B) or laterally (C). D – L. Whole mount in situ hybridizations of stage 45 dissected brains using probes for insm1 (D – F), foxn4 (G – I), or lhx5 (J – L). insm1 is expressed in the dorsal pallium, optic tectum and rhombomeres as viewed dorsally (D). insm1 expression was also detected ventrally (E) and laterally (F) in the hypothalamus, preoptic area and isthmus. Scale bars (1 μM) are indicated in (D) for (D – F), (G) for (G – I) and (J) for (J – L). Abbreviations: DP – dorsal pallium, H – hypothalamus, IS – isthmus, OB – olfactory bulb, OT – optic tectum, PO – preoptic area, RF – reticular formation, R – rhombomeres.
	insm1 (insulinoma associated 1) gene expression in Xenopus laevis brain (isolated), assayed via in situ hybridization, NF stage 45, lateral view, anterior right, dorsal up. Key: H = hypothalamus, OT = optic tectum, PO =preoptic area.
	insm1 (insulinoma associated 1) gene expression in sections of Xenopus laevis eye, assayed via in situ hybridization, NF stage 45.

Akerstrom, Adenoviral insulinoma-associated protein 1 promoter-driven suicide gene therapy with enhanced selectivity for treatment of neuroendocrine cancers. 2013, Pubmed

Akerstrom, Adenoviral insulinoma-associated protein 1 promoter-driven suicide gene therapy with enhanced selectivity for treatment of neuroendocrine cancers. 2013, Pubmed
Bachy, The LIM-homeodomain gene family in the developing Xenopus brain: conservation and divergences with the mouse related to the evolution of the forebrain. 2001, Pubmed , Xenbase
Bailey, Regulation of vertebrate eye development by Rx genes. 2004, Pubmed , Xenbase
Breslin, NeuroD1/E47 regulates the E-box element of a novel zinc finger transcription factor, IA-1, in developing nervous system. 2003, Pubmed
Christensen, Targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay. 2010, Pubmed
Coffman, Xotch, the Xenopus homolog of Drosophila notch. 1990, Pubmed , Xenbase
Colombo, Mouse orthologue of ARX, a gene mutated in several X-linked forms of mental retardation and epilepsy, is a marker of adult neural stem cells and forebrain GABAergic neurons. 2004, Pubmed
Duggan, Transient expression of the conserved zinc finger gene INSM1 in progenitors and nascent neurons throughout embryonic and adult neurogenesis. 2008, Pubmed
El-Hodiri, The Xenopus arx gene is expressed in the developing rostral forebrain. 2003, Pubmed , Xenbase
Forbes-Osborne, Insulinoma-associated 1a (Insm1a) is required for photoreceptor differentiation in the zebrafish retina. 2013, Pubmed
Gierl, The zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic beta cells and intestinal endocrine cells. 2006, Pubmed
Goto, A novel human insulinoma-associated cDNA, IA-1, encodes a protein with "zinc-finger" DNA-binding motifs. 1992, Pubmed
Jacob, Insm1 (IA-1) is an essential component of the regulatory network that specifies monoaminergic neuronal phenotypes in the vertebrate hindbrain. 2009, Pubmed
Kelly, Expression of the forkhead transcription factor FoxN4 in progenitor cells in the developing Xenopus laevis retina and brain. 2006, Pubmed , Xenbase
Lan, IA-1, a new marker for neuroendocrine differentiation in human lung cancer cell lines. 1993, Pubmed
Lee, Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein. 1995, Pubmed , Xenbase
Martinez-De Luna, The Retinal Homeobox (Rx) gene is necessary for retinal regeneration. 2011, Pubmed , Xenbase
Mellitzer, IA1 is NGN3-dependent and essential for differentiation of the endocrine pancreas. 2006, Pubmed
Moreno, LIM-homeodomain genes as territory markers in the brainstem of adult and developing Xenopus laevis. 2005, Pubmed , Xenbase
Moreno, LIM-homeodomain genes as developmental and adult genetic markers of Xenopus forebrain functional subdivisions. 2004, Pubmed , Xenbase
Morris, Microarray analysis of XOPS-mCFP zebrafish retina identifies genes associated with rod photoreceptor degeneration and regeneration. 2011, Pubmed
Nelson, Transient inactivation of Notch signaling synchronizes differentiation of neural progenitor cells. 2007, Pubmed
Parlier, Xenopus zinc finger transcription factor IA1 (Insm1) expression marks anteroventral noradrenergic neuron progenitors in Xenopus embryos. 2008, Pubmed , Xenbase
Pearl, Xenopus pancreas development. 2009, Pubmed , Xenbase
Pedersen, The insulinoma-associated 1: a novel promoter for targeted cancer gene therapy for small-cell lung cancer. 2006, Pubmed
Perron, The genetic sequence of retinal development in the ciliary margin of the Xenopus eye. 1998, Pubmed , Xenbase
Ramachandran, Insm1a-mediated gene repression is essential for the formation and differentiation of Müller glia-derived progenitors in the injured retina. 2012, Pubmed
Rosenbaum, Insm1 promotes the transition of olfactory progenitors from apical and proliferative to basal, terminally dividing and neuronogenic. 2011, Pubmed
Viczian, XOtx5b and XOtx2 regulate photoreceptor and bipolar fates in the Xenopus retina. 2003, Pubmed , Xenbase
Wang, INSM1 promoter-driven adenoviral herpes simplex virus thymidine kinase cancer gene therapy for the treatment of primitive neuroectodermal tumors. 2010, Pubmed
Yoshii, Neural retinal regeneration in the anuran amphibian Xenopus laevis post-metamorphosis: transdifferentiation of retinal pigmented epithelium regenerates the neural retina. 2007, Pubmed , Xenbase