Jacox L et al. (2016), Formation of a "Pre-mouth Array" from the Extre...

XB-ART-52331

Cell Rep 2016 Aug 02;165:1445-1455. doi: 10.1016/j.celrep.2016.06.073.

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Formation of a "Pre-mouth Array" from the Extreme Anterior Domain Is Directed by Neural Crest and Wnt/PCP Signaling.

Jacox L , Chen J , Rothman A , Lathrop-Marshall H , Sive H .

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The mouth arises from the extreme anterior domain (EAD), a region where the ectoderm and endoderm are directly juxtaposed. Here, we identify a "pre-mouth array" in Xenopus that forms soon after the cranial neural crest has migrated to lie on either side of the EAD. Initially, EAD ectoderm comprises a wide and short epithelial mass that becomes narrow and tall with cells and nuclei changing shape, a characteristic of convergent extension. The resulting two rows of cells-the pre-mouth array-later split down the midline to surround the mouth opening. Neural crest is essential for convergent extension and likely signals to the EAD through the Wnt/planar cell polarity (PCP) pathway. Fzl7 receptor is locally required in EAD ectoderm, while Wnt11 ligand is required more globally. Indeed, heterologous cells expressing Wnt11 can elicit EAD convergent extension. The study reveals a precise cellular mechanism that positions and contributes to the future mouth.

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Species referenced: Xenopus laevis
Genes referenced: dvl2 frzb fzd7 mapk8 mmp11 pitx1 pitx2 rac1 rho sox9 wnt11 wnt11b
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	Figure 1. Coronal Anatomy of Xenopus Face and EAD Ectoderm between Late Neurula and Swimming Tadpole (A and O) Schematic, stages 22–28 and 35/36–40. (B–E′ and P–S′) Coronal sections with β-catenin immunolabeling (two independent experiments; stage 22, n = 10; stage 24, n = 11; stage 26, n = 14; stage 28, n = 14). Midline region (bracket) with bright β-catenin labeling is EAD ectoderm of the pre-mouth array. Bracket: region of 10× image (B–E and P–S) enlarged in 25× view (B′–E′ and P′–S′). Asterisk in (E), pre-mouth array at stage 28, enlarged in (E′). cg, cement gland. (F–I and T–W) Still frames from Claymation of mouth opening found in Movie S2. (I) Pre-mouth array stage of Claymation. (J–M′ and X–a′) Coronal sections with laminin (green) immunolabeling with propidium iodide (PI) nuclear counterstain (red) (two independent experiments; stage 22, n = 10; stage 24, n = 4; stage 26, n = 6; stage 28, n = 7). Bracket: region of 10× image (J–M and X–a) enlarged in 25× view (J′–M′ and X′–a′). Asterisk (M), pre-mouth array at stage 28, enlarged in (M′). (B′, C′, J′, and K′) White boxes surround lateral regions next to EAD, which fill with NC cells between stages 22 (B′ and J′) and 24 (C′ and K′). Scale bars represent 170 μm (10×) and 68 μm (25×).
	Figure 2. Detailed Anatomy and Modeling of Xenopus EAD Ectoderm between Late Neurula and Swimming Tadpole (A–D′) Coronal sections with β-catenin (green) immunolabeling with PI nuclear counterstain (red) from stages 22–28 (two independent experiments; stage 22, n = 10; stage 24, n = 11; stage 26, n = 14; stage 28 n = 14). (E–H′) Sagittal sections with β-catenin (green) immunolabeling from stages 22–28 (three independent experiments; stage 22, n = 12; stage 24, n = 10; stage 26, n = 12; stage 28, n = 12). (A′–D′ and E′–H′) Cell membranes traced in white. Blue line separates deep EAD from outer ectoderm. Yellow line separates EAD ectoderm from endoderm. (A′′–D′′ and E′′–H′′) Cell outlines in black. Dotted line is the top of the cement gland (cg). (D–D′′) Pre-mouth array is present and indicated by cell outlines. Scale bar (25×), 68 μm. Scale bars (40×), 43 μm. (I and J) Model 1. (I) Stage 22. (J) Stage 28. (K and L) Model 2. (K) Stage 22. (L) Stage 28. (M) Quantification of height versus width of EAD (see Experimental Procedures) (three independent experiments; stage 22, n = 12; stage 24, n = 16; stage 26, n = 17; stage 28, n = 47). p values were obtained from unpaired, two-tailed t tests comparing sequential stages. Error bar represents SD. (N) Diagram demonstrating coronal (A–D′) and sagittal (E–H′) sections. (O) Diagram showing the change in height (H), width (W), and depth (D) of the EAD ectoderm and its surrounding BM between stages 22 and 28. Blue ovals, EAD ectodermal cells undergoing convergent extension. Orange rectangular prism, laminin BM surrounding EAD ectoderm.
	Figure 3. EAD Ectoderm Undergoes Convergent Extension as the Cranial Neural Crest Approaches the Midline and EAD Convergent Extension Fails to Occur in sox9 LOF Embryos (A) Experimental schematic. (B–D′) Coronal sections with mGFP-labeled NC (green) and β-catenin (red) immunolabeling from late neurula (stage 24) to late tailbud (stage 28) (two independent experiments; stage 24, n = 7; stage 26, n = 7; stage 28, n = 4). Midline region (bracket) with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (B–D) enlarged in 40× view (B′–D′) and 63× view (B′′–D′′). cg, cement gland. (E and F) Frontal view of control and sox9 LOF embryos at swimming tadpole (stage 40) assayed in two experiments (control MO in E, n = 24; sox9 MO in F, n = 30.) Dots surround open mouth. Bracket: unopened mouth. Scale bar, 200 μm. (G–H′) Coronal sections assayed in four independent experiments (n = 23) with β-catenin immunolabeling and Hoechst nuclear labeling. Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (G and H) enlarged in 25× view (G′ and H′). cg, cement gland. (I) Graph depicting percent of embryos displaying face, mouth, nostril, and pigment formation phenotypes at stage 40 in control and sox9 LOF embryos. p values were obtained from Fisher’s exact probability test. (J) Quantification of height over width of EAD (see Experimental Procedures). p values were obtained from unpaired, two-tailed t tests. Error bar represents SD. Unless otherwise specified, scale bars represent 170 μm (10×), 68 μm (25×), 43 μm (40×), and 27 μm (63×).
	Figure 4. Fzl7 Is Locally Required in the EAD Ectoderm for Convergent Extension Local requirement of Dsh, fzl7, and wnt11 expression tested with an EAD transplant technique. (A) Experimental design: donor LOF tissue was transplanted to uninjected sibling recipients. (B–C′) EAD transplant outcome from control or Dep+ RNA donor tissue assayed in three experiments. (B and B′) Control RNA (n = 23). (C and C′) Dep+ RNA (n = 22). (B′–C′) Overlay of (B) and (C) with GFP fluorescence indicating location of donor transplant in recipient. Dots surround open mouths. Bracket: unopened mouth. Frontal view. cg, cement gland. Scale bar, 200 μm. (D–E′) Coronal sections of EAD transplants with control or Dep+ donor tissue assayed in three independent experiments. (D and D′) Control RNA (n = 10); (E and E′) Dep+ RNA (n = 14) with β-catenin immunolabeling. Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (D and E) enlarged in 25× view (D′–E′). (F) Quantification of normal or abnormal structure development depending on background of facial tissue. p values were obtained from Fisher’s exact probability test. (G) Quantification of height over width of EAD (see Experimental Procedures). p values are from unpaired, two-tailed t tests. Error bar represents SD. (H–J) EAD transplant outcome from control, fzl7, or wnt11 LOF donor tissue assayed in four independent experiments. (H and H′) Control MO (n = 27). (I and I′) fzl7 MO (n = 30). (J and J′) wnt11 MO (n = 30). (H′–J′) Overlay of (H)–(J) with GFP fluorescence indicating location of donor transplant in recipient. Dots surround open mouths. Bracket: unopened mouth. Frontal view. Scale bar, 200 μm. (K–M′) Coronal sections of EAD transplants with control, fzl7, or wnt11 donor tissue assayed in four independent experiments with β-catenin immunolabeling. (K and K′) Control MO (n = 19). (L and L ′) fzl7 MO (n = 17). (M and M′) wnt11 MO (n = 14). Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (K–M) enlarged in 25× view (K′–M′). (N) Quantification of normal or abnormal structure development depending on LOF background of facial tissue. p values were obtained from Fisher’s exact probability test. (O) Quantification of height over width of EAD midline tissue in transplants. p values are from unpaired, two-tailed t tests. (P) Schematic of model. NC releases Wnt11, which acts on Fzl7 receptors expressed on midline EAD cells. Unless otherwise specified, scale bars represent 170 μm (10×) and 68 μm (25×).
	Figure 5. Inhibition of GTPases JNK and Rac1 Is Associated with a Reduction in EAD Ectodermal Convergent Extension (A) Experimental schematic of inhibitor loaded bead implantation in the presumptive mouth, EAD region. (B–F) Frontal view of swimming tadpole (stage 40) embryos with inhibitor loaded beads implanted in presumptive mouths, assayed in three experiments. (B) Control DMSO (n = 97). (C) Rac1 (n = 40). (D) JNK inhibitor (n = 44). (E) Rock inhibitor (n = 75). (F) Rho inhibitor (n = 39). Bracket: unopened mouth. Dots surround open mouths. cg, cement gland. Scale bar, 200 μm. (G-K′) Coronal sections, stage 28, assayed in 3 independent experiments. (G and G′) Control DMSO (n = 31). (H and H′) Rac1 inhibitor (n = 27). (I and I′) JNK inhibitor (n = 24). (J and J′) Rock inhibitor (n = 9). (K and K′) Rho inhibitor (n = 12) with β-catenin immunolabeling. Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (G–K) enlarged in 25× view (G′–K′). Scale bars represent 170 μm (10×) and 68 μm (25×). (L) Graph depicting percentage of embryos, displaying face, mouth, nostril, and pigment formation phenotypes at stage 40. p values are from Fisher’s exact probability test. (M) Quantification of height over width of EAD (see Experimental Procedures). p values are from unpaired, two-tailed t tests. Error bar represents SD. (N–S′) Control and fzl7 LOF embryos at stage 20 (N and N′, n = 19; Q and Q′, n = 26), stage 23 (O and O′, n = 23; R and R′, n = 23), and stage 26 (P and P′, n = 21; S and S′, n = 16) with p-JNK immunolabeling (green), mApple cell membranes (red), and Hoechst nuclear counterstain (blue in N–S) assayed in two experiments. Bracket: EAD. Scale bars (40×), 43 μm. (T) Quantification of cells with p-JNK positive nuclei in the EAD ectoderm. The total number of EAD nuclei was equivalent between stage-matched control and fzl7 LOF embryos. p < 0.0006, control stage 23 compared to stages 20 and 26. p values were obtained from unpaired, two-tailed t tests. (U–W′) Coronal sections, stage 28, assayed in two independent experiments. (U and U′) Control water (n = 20). (V and V′) wnt11 MO + control water (n = 30). (W and W′) wnt11 MO + anisomycin JNK activator (n = 21) with β-catenin immunolabeling. Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (U–W) enlarged in 25× view (U′ –W′). Scale bars represent 170 μm (10×) and 68 μm (25×). (X) Quantification of height over width of EAD (see Experimental Procedures). Error bar represents SD. p values are from unpaired, two-tailed t tests.
	Figure 6. Wnt11 Is Sufficient for EAD Ectoderm Convergent Extension (A) Sufficiency of Wnt11 for midline convergent extension (CE) was tested with an animal cap transplant technique. Experimental schematic of bilateral transplants with mApple, animal cap overexpressing Wnt11 or a control, secreted protein (inactive MMP11). (B–E) Overlay of bright-field images with mApple fluorescence indicating location of donor transplant in late tailbud recipients (stage 28). Scale bar, 200 μm. (F–I′) Coronal sections of animal cap transplants with mmp11 or wnt11 overexpressing donor tissue assayed in three experiments with β-catenin immunolabeling. (F, F′) Control MO + mmp11 (n = 15). (G and G′) Control MO + wnt11 (n = 14). (H and H′) sox9 MO + mmp11 (n = 18). (I and I′) sox9 MO + wnt11 (n = 22). Midline region with bright β-catenin labeling is EAD ectoderm. Bracket: region of 10× image (F–I) enlarged in 25× view (F′ –I′). (J) Quantification of height over width of EAD (see Experimental Procedures). p values are from unpaired, two-tailed t tests. Error bar represents SD. Unless otherwise specified, scale bars represent 170 μm (10×) and 68 μm (25×).

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