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J Dev Biol 2016 Mar 01;41:. doi: 10.3390/jdb4010002.
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Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis.

Aguero T , Zhou Y , Kloc M , Chang P , Houliston E , King ML .

The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline function of blocking somatic gene expression and thus preventing Primordial Germ Cell (PGC) loss and sterility. Hermes/Rbpms protein and nanos RNA co-localize within germinal granules, diagnostic electron dense particles found within the germ plasm. Previous work indicates that nanos accumulates within the germ plasm through a diffusion/entrapment mechanism. Here we show that Hermes/Rbpms interacts with nanos through sequence specific RNA localization signals found in the nanos-3'UTR. Importantly, Hermes/Rbpms specifically binds nanos, but not Vg1 RNA in the nucleus of stage I oocytes. In vitro binding data show that Hermes/Rbpms requires additional factors that are present in stage I oocytes in order to bind nanos1. One such factor may be hnRNP I, identified in a yeast-2-hybrid screen as directly interacting with Hermes/Rbpms. We suggest that Hermes/Rbpms functions as part of a RNP complex in the nucleus that facilitates selection of germline RNAs for germ plasm localization. We propose that Hermes/Rbpms is required for nanos RNA to form within the germinal granules and in this way, participates in the germline specific translational repression and sequestration of nanos RNA.

PubMed ID: 26998427
PMC ID: PMC4798249
Article link: J Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: gdf1 hnrnpc hnrnpdl hnrnpl hsp90b1 igf2bp3 lgals4.2 myc nanos1 npm1 pam pgat pigy ptbp1 rbpms rbpms2 stau1 tbl1x vegt
Antibodies: Npm1 Ab1 Tuba1a Ab1

Article Images: [+] show captions
References [+] :
Beckhelling, Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts. 2003, Pubmed, Xenbase