Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Int Rev Cell Mol Biol
2014 Jan 01;313:103-44. doi: 10.1016/B978-0-12-800177-6.00004-9.
Show Gene links
Show Anatomy links
The UNC-45 myosin chaperone: from worms to flies to vertebrates.
Lee CF
,
Melkani GC
,
Bernstein SI
.
???displayArticle.abstract???
UNC-45 (uncoordinated mutant number 45) is a UCS (UNC-45, CRO1, She4p) domain protein that is critical for myosin stability and function. It likely aides in folding myosin during cellular differentiation and maintenance, and protects myosin from denaturation during stress. Invertebrates have a single unc-45 gene that is expressed in both muscle and nonmuscle tissues. Vertebrates possess one gene expressed in striated muscle (unc-45b) and another that is more generally expressed (unc-45a). Structurally, UNC-45 is composed of a series of α-helices connected by loops. It has an N-terminal tetratricopeptide repeat domain that binds to Hsp90 and a central domain composed of armadillo repeats. Its C-terminal UCS domain, which is also comprised of helical armadillo repeats, interacts with myosin. In this chapter, we present biochemical, structural, and genetic analyses of UNC-45 in Caenorhabditis elegans, Drosophila melanogaster, and various vertebrates. Further, we provide insights into UNC-45 functions, its potential mechanism of action, and its roles in human disease.
Anderson,
Loss of unc45a precipitates arteriovenous shunting in the aortic arches.
2008, Pubmed
Anderson,
Loss of unc45a precipitates arteriovenous shunting in the aortic arches.
2008,
Pubmed
Ao,
Caenorhabditis elegans UNC-45 is a component of muscle thick filaments and colocalizes with myosin heavy chain B, but not myosin heavy chain A.
2000,
Pubmed
Barral,
Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin.
2002,
Pubmed
Barral,
Unc-45 mutations in Caenorhabditis elegans implicate a CRO1/She4p-like domain in myosin assembly.
1998,
Pubmed
Bazzaro,
Myosin II co-chaperone general cell UNC-45 overexpression is associated with ovarian cancer, rapid proliferation, and motility.
2007,
Pubmed
Beach,
ASH1 mRNA localization in three acts.
2001,
Pubmed
Bernick,
Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos.
2010,
Pubmed
Berteaux-Lecellier,
A homologue of the yeast SHE4 gene is essential for the transition between the syncytial and cellular stages during sexual reproduction of the fungus Podospora anserina.
1998,
Pubmed
Bird,
Chaperone-enhanced purification of unconventional myosin 15, a molecular motor specialized for stereocilia protein trafficking.
2014,
Pubmed
Bujalowski,
UNC-45B chaperone: the role of its domains in the interaction with the myosin motor domain.
2014,
Pubmed
Chadli,
GCUNC45 is the first Hsp90 co-chaperone to show alpha/beta isoform specificity.
2008,
Pubmed
Chadli,
GCUNC-45 is a novel regulator for the progesterone receptor/hsp90 chaperoning pathway.
2006,
Pubmed
Chadli,
Analysis of Hsp90 cochaperone interactions reveals a novel mechanism for TPR protein recognition.
2008,
Pubmed
Chen,
Dual function of the UNC-45b chaperone with myosin and GATA4 in cardiac development.
2012,
Pubmed
Comyn,
Lack of developmental redundancy between Unc45 proteins in zebrafish muscle development.
2012,
Pubmed
D'Andrea,
TPR proteins: the versatile helix.
2003,
Pubmed
Du,
Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos.
2008,
Pubmed
Epping,
UNC45A confers resistance to histone deacetylase inhibitors and retinoic acid.
2009,
Pubmed
Epstein,
Temperature-sensitive mutation affecting myofilament assembly in Caenorhabditis elegans.
1974,
Pubmed
Estrada,
An integrated strategy for analyzing the unique developmental programs of different myoblast subtypes.
2006,
Pubmed
Etard,
Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril.
2008,
Pubmed
Etard,
Lack of Apobec2-related proteins causes a dystrophic muscle phenotype in zebrafish embryos.
2010,
Pubmed
Etard,
The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis.
2007,
Pubmed
Etheridge,
A zebrafish unc-45-related gene expressed during muscle development.
2002,
Pubmed
Fratev,
Structural insight into the UNC-45-myosin complex.
2013,
Pubmed
Gaiser,
Downregulation of the Hsp90 system causes defects in muscle cells of Caenorhabditis elegans.
2011,
Pubmed
Gazda,
The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.
2013,
Pubmed
Geach,
Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker.
2010,
Pubmed
,
Xenbase
Groves,
Topological characteristics of helical repeat proteins.
1999,
Pubmed
Guo,
Differential turnover of myosin chaperone UNC-45A isoforms increases in metastatic human breast cancer.
2011,
Pubmed
Hansen,
The myosin chaperone UNC45B is involved in lens development and autosomal dominant juvenile cataract.
2014,
Pubmed
Hawkins,
The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis.
2008,
Pubmed
Hoppe,
Regulation of the myosin-directed chaperone UNC-45 by a novel E3/E4-multiubiquitylation complex in C. elegans.
2004,
Pubmed
Hutagalung,
The UCS family of myosin chaperones.
2002,
Pubmed
Janiesch,
The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathy.
2007,
Pubmed
Ju,
Inclusion body myopathy, Paget's disease of the bone and fronto-temporal dementia: a disorder of autophagy.
2010,
Pubmed
Kachur,
Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle myosin in the early Caenorhabditis elegans embryo.
2004,
Pubmed
Kachur,
UNC-45 is required for NMY-2 contractile function in early embryonic polarity establishment and germline cellularization in C. elegans.
2008,
Pubmed
Kaiser,
Tracking UNC-45 chaperone-myosin interaction with a titin mechanical reporter.
2012,
Pubmed
Kuo,
GATA4 transcription factor is required for ventral morphogenesis and heart tube formation.
1997,
Pubmed
Kwartler,
Overexpression of smooth muscle myosin heavy chain leads to activation of the unfolded protein response and autophagic turnover of thick filament-associated proteins in vascular smooth muscle cells.
2014,
Pubmed
Landsverk,
The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans.
2007,
Pubmed
Lee,
Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stability.
2011,
Pubmed
Lee,
X-ray crystal structure of the UCS domain-containing UNC-45 myosin chaperone from Drosophila melanogaster.
2011,
Pubmed
Li,
Smyd1b is required for skeletal and cardiac muscle function in zebrafish.
2013,
Pubmed
Liu,
Unc45 activates Hsp90-dependent folding of the myosin motor domain.
2008,
Pubmed
Lord,
UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II.
2004,
Pubmed
Lord,
Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells.
2008,
Pubmed
Melkani,
Drosophila UNC-45 prevents heat-induced aggregation of skeletal muscle myosin and facilitates refolding of citrate synthase.
2010,
Pubmed
Melkani,
The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model.
2011,
Pubmed
Melkani,
Huntington's disease induced cardiac amyloidosis is reversed by modulating protein folding and oxidative stress pathways in the Drosophila heart.
2013,
Pubmed
Mishra,
Hsp90 protein in fission yeast Swo1p and UCS protein Rng3p facilitate myosin II assembly and function.
2005,
Pubmed
Myhre,
Unc45b is essential for early myofibrillogenesis and costamere formation in zebrafish.
2014,
Pubmed
Ni,
The myosin-binding UCS domain but not the Hsp90-binding TPR domain of the UNC-45 chaperone is essential for function in Caenorhabditis elegans.
2011,
Pubmed
Price,
Two mammalian UNC-45 isoforms are related to distinct cytoskeletal and muscle-specific functions.
2002,
Pubmed
Scheufler,
Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine.
2000,
Pubmed
Shi,
UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region.
2010,
Pubmed
Srikakulam,
Chaperone-mediated folding and assembly of myosin in striated muscle.
2004,
Pubmed
Srikakulam,
Unc45b forms a cytosolic complex with Hsp90 and targets the unfolded myosin motor domain.
2008,
Pubmed
Srikakulam,
Myosin II folding is mediated by a molecular chaperonin.
1999,
Pubmed
Tewari,
Armadillo-repeat protein functions: questions for little creatures.
2010,
Pubmed
Toi,
She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae.
2003,
Pubmed
Venolia,
The unc-45 gene of Caenorhabditis elegans is an essential muscle-affecting gene with maternal expression.
1990,
Pubmed
Venolia,
unc-45 gene of Caenorhabditis elegans encodes a muscle-specific tetratricopeptide repeat-containing protein.
1999,
Pubmed
Wesche,
The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function.
2003,
Pubmed
Wohlgemuth,
The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
2007,
Pubmed
Wong,
Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis.
2000,
Pubmed
Zeisberg,
Morphogenesis of the right ventricle requires myocardial expression of Gata4.
2005,
Pubmed
