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J Cell Sci 2015 Mar 01;1285:888-99. doi: 10.1242/jcs.155499.
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TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment in Xenopus.

Futel M , Leclerc C , Le Bouffant R , Buisson I , Néant I , Umbhauer M , Moreau M , Riou JF .

In Xenopus laevis embryos, kidney field specification is dependent on retinoic acid (RA) and coincides with a dramatic increase of Ca(2+) transients, but the role of Ca(2+) signaling in the kidney field is unknown. Here, we identify TRPP2, a member of the transient receptor potential (TRP) superfamily of channel proteins encoded by the pkd2 gene, as a central component of Ca(2+) signaling in the kidney field. TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular Ca(2+) entry. Knockdown of pkd2 in the kidney field results in the downregulation of pax8, but not of other kidney field genes (lhx1, osr1 and osr2). We further show that inhibition of Ca(2+) signaling with an inducible Ca(2+) chelator also causes downregulation of pax8, and that pkd2 knockdown results in a severe inhibition of Ca(2+) transients in kidney field explants. Finally, we show that disruption of RA results both in an inhibition of intracellular Ca(2+) signaling and of TRPP2 incorporation into the plasma membrane of kidney field cells. We propose that TRPP2-dependent Ca(2+) signaling is a key component of pax8 regulation in the kidney field downstream of RA-mediated non-transcriptional control of TRPP2.

PubMed ID: 25588842
Article link: J Cell Sci

Species referenced: Xenopus laevis
Genes referenced: arhgef7 arl13b cyp26a1 klf6 lhx1 myod1 osr1 osr2 pax8 pkd2 pmt prlhr st14 tbx2
Antibodies: Pkd2 Ab1
Morpholinos: pkd2 MO1

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