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Methods Enzymol
2014 Jan 01;546:355-75. doi: 10.1016/B978-0-12-801185-0.00017-9.
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Cas9-based genome editing in Xenopus tropicalis.
Nakayama T
,
Blitz IL
,
Fish MB
,
Odeleye AO
,
Manohar S
,
Cho KW
,
Grainger RM
.
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Xenopus tropicalis has been developed as a model organism for developmental biology, providing a system offering both modern genetics and classical embryology. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) system for genome modification has provided an additional tool for Xenopus researchers to achieve simple and efficient targeted mutagenesis. Here, we provide insights into experimental design and procedures permitting successful application of this technique to Xenopus researchers, and offer a general strategy for performing loss-of-function assays in F0 and subsequently F1 embryos.
Abu-Daya,
The hitchhiker's guide to Xenopus genetics.
2012, Pubmed,
Xenbase
Abu-Daya,
The hitchhiker's guide to Xenopus genetics.
2012,
Pubmed
,
Xenbase
Anders,
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease.
2014,
Pubmed
Ansai,
Targeted mutagenesis using CRISPR/Cas system in medaka.
2014,
Pubmed
Baklanov,
Effect on DNA transcription of nucleotide sequences upstream to T7 promoter.
1996,
Pubmed
Blitz,
Biallelic genome modification in F(0) Xenopus tropicalis embryos using the CRISPR/Cas system.
2013,
Pubmed
,
Xenbase
Carroll,
Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase
Chen,
Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.
2013,
Pubmed
Cradick,
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity.
2013,
Pubmed
Fineran,
Gene regulation by engineered CRISPR-Cas systems.
2014,
Pubmed
Fish,
Xenopus mutant reveals necessity of rax for specifying the eye field which otherwise forms tissue with telencephalic and diencephalic character.
2014,
Pubmed
,
Xenbase
Fu,
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.
2014,
Pubmed
Fu,
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.
2013,
Pubmed
Guilinger,
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification.
2014,
Pubmed
Guo,
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
2014,
Pubmed
,
Xenbase
Guschin,
A rapid and general assay for monitoring endogenous gene modification.
2010,
Pubmed
Harland,
Xenopus research: metamorphosed by genetics and genomics.
2011,
Pubmed
,
Xenbase
Hirsch,
Xenopus tropicalis transgenic lines and their use in the study of embryonic induction.
2002,
Pubmed
,
Xenbase
Hsu,
Development and applications of CRISPR-Cas9 for genome engineering.
2014,
Pubmed
Hsu,
DNA targeting specificity of RNA-guided Cas9 nucleases.
2013,
Pubmed
Hwang,
Efficient genome editing in zebrafish using a CRISPR-Cas system.
2013,
Pubmed
Ishibashi,
Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis.
2012,
Pubmed
,
Xenbase
Kim,
Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.
2009,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Lei,
Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs).
2012,
Pubmed
,
Xenbase
Mali,
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
2013,
Pubmed
Mali,
RNA-guided human genome engineering via Cas9.
2013,
Pubmed
Nakajima,
Targeted gene disruption in the Xenopus tropicalis genome using designed TALE nucleases.
2013,
Pubmed
,
Xenbase
Nakajima,
Generation of albino Xenopus tropicalis using zinc-finger nucleases.
2012,
Pubmed
,
Xenbase
Nakajima,
Comparison of TALEN scaffolds in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Nakayama,
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Ogino,
High-throughput transgenesis in Xenopus using I-SceI meganuclease.
2006,
Pubmed
,
Xenbase
Olson,
Maternal mRNA knock-down studies: antisense experiments using the host-transfer technique in Xenopus laevis and Xenopus tropicalis.
2012,
Pubmed
,
Xenbase
Pattanayak,
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.
2013,
Pubmed
Ran,
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.
2013,
Pubmed
Sander,
CRISPR-Cas systems for editing, regulating and targeting genomes.
2014,
Pubmed
Suzuki,
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
2013,
Pubmed
,
Xenbase
Terns,
CRISPR-based technologies: prokaryotic defense weapons repurposed.
2014,
Pubmed
Tsai,
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
2014,
Pubmed
Wang,
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.
2013,
Pubmed
Wittwer,
High-resolution genotyping by amplicon melting analysis using LCGreen.
2003,
Pubmed
Yang,
One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.
2013,
Pubmed
Young,
Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases.
2011,
Pubmed
,
Xenbase
