Zariwala MA , Gee HY , Kurkowiak M , Al-Mutairi DA , Leigh MW , Hurd TW , Hjeij R , Dell SD , Chaki M , Dougherty GW , Adan M , Spear PC , Esteve-Rudd J , Loges NT , Rosenfeld M , Diaz KA , Olbrich H , Wolf WE , Sheridan E , Batten TF , Halbritter J , Porath JD , Kohl S , Lovric S , Hwang DY , Pittman JE , Burns KA , Ferkol TW , Sagel SD , Olivier KN , Morgan LC , Werner C , Raidt J , Pennekamp P , Sun Z , Zhou W , Airik R , Natarajan S , Allen SJ , Amirav I , Wieczorek D , Landwehr K , Nielsen K , Schwerk N , Sertic J , Köhler G , Washburn J , Levy S , Fan S , Koerner-Rettberg C , Amselem S , Williams DS , Mitchell BJ , Drummond IA , Otto EA , Omran H , Knowles MR , Hildebrandt F .

5 R01HL071798 NHLBI NIH HHS , 5 U54 HL096458-06 NHLBI NIH HHS , DK053093 NIDDK NIH HHS , DK068306 NIDDK NIH HHS , DK070263 NIDDK NIH HHS , DK090917 NIDDK NIH HHS , DK091405 NIDDK NIH HHS , DK092808-01A1 NIDDK NIH HHS , EY013408 NEI NIH HHS , UL1 TR000083 NCATS NIH HHS , UL1 TR000154 NCATS NIH HHS , Howard Hughes Medical Institute , P30 EY000331 NEI NIH HHS , R00 DK091405 NIDDK NIH HHS , R01 DK068306 NIDDK NIH HHS , R01 EY013408 NEI NIH HHS , R01 GM089970 NIGMS NIH HHS , R01 HL071798 NHLBI NIH HHS , U54 RR019480 NCRR NIH HHS , R01 DK053093 NIDDK NIH HHS , R01 DK070263 NIDDK NIH HHS , U54 HL096458 NHLBI NIH HHS , R01 DK092808 NIDDK NIH HHS , RC4 DK090917 NIDDK NIH HHS , K99 DK091405 NIDDK NIH HHS

Figure S6. Morpholino Knockdown Phenotype and Localization of zmynd10 ( in zebrfish) (A-C) Wild-type and zmynd10 morphant embryos stained with the cilia marker acetylated B-tubulin (green) and the actin marker phalloidin (red). Both the ATG and splice (SPL) morpholinos result in a substantial decrease in cilia number relative to control cells. (D) Quantification of cilia-generated fluid flow in wild type and morphant embryos. (**** P < 0.0001). (E) Co-localization of GFP-centrin4 (green) with RFP-hsLRRC6 (red) at the basal bodies of Xenopus multi-ciliated cells. (F) Localization of GFP-hsZMYND10 (green) to the basal bodies marked with Centrin4-RFP (red) as well as to the striated rootlets that project away from the basal bodies.

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