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Plant Physiol
2013 Nov 01;1633:1338-52. doi: 10.1104/pp.113.224972.
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Overexpression of the vacuolar sugar carrier AtSWEET16 modifies germination, growth, and stress tolerance in Arabidopsis.
Klemens PA
,
Patzke K
,
Deitmer J
,
Spinner L
,
Le Hir R
,
Bellini C
,
Bedu M
,
Chardon F
,
Krapp A
,
Neuhaus HE
.
Abstract
Here, we report that SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEET16) from Arabidopsis (Arabidopsis thaliana) is a vacuole-located carrier, transporting glucose (Glc), fructose (Fru), and sucrose (Suc) after heterologous expression in Xenopus laevis oocytes. The SWEET16 gene, similar to the homologs gene SWEET17, is mainly expressed in vascular parenchyma cells. Application of Glc, Fru, or Suc, as well as cold, osmotic stress, or low nitrogen, provoke the down-regulation of SWEET16 messenger RNA accumulation. SWEET16 overexpressors (35SPro:SWEET16) showed a number of peculiarities related to differences in sugar accumulation, such as less Glc, Fru, and Suc at the end of the night. Under cold stress, 35SPro:SWEET16 plants are unable to accumulate Fru, while under nitrogen starvation, both Glc and Fru, but not Suc, were less abundant. These changes of individual sugars indicate that the consequences of an increased SWEET16 activity are dependent upon the type of external stimulus. Remarkably, 35SPro:SWEET16 lines showed improved germination and increased freezing tolerance. The latter observation, in combination with the modified sugar levels, points to a superior function of Glc and Suc for frost tolerance. 35SPro:SWEET16 plants exhibited increased growth efficiency when cultivated on soil and showed improved nitrogen use efficiency when nitrate was sufficiently available, while under conditions of limiting nitrogen, wild-type biomasses were higher than those of 35SPro:SWEET16 plants. Our results identify SWEET16 as a vacuolar sugar facilitator, demonstrate the substantial impact of SWEET16 overexpression on various critical plant traits, and imply that SWEET16 activity must be tightly regulated to allow optimal Arabidopsis development under nonfavorable conditions.
Aluri,
Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering.
2007, Pubmed
Aluri,
Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering.
2007,
Pubmed
Arenas-Huertero,
Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar.
2000,
Pubmed
Bröer,
Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes.
1999,
Pubmed
,
Xenbase
Büttner,
Monosaccharide transporters in plants: structure, function and physiology.
2000,
Pubmed
Chardon,
Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply.
2010,
Pubmed
Chardon,
Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis.
2013,
Pubmed
,
Xenbase
Chen,
Sugar transporters for intercellular exchange and nutrition of pathogens.
2010,
Pubmed
,
Xenbase
Chen,
Sucrose efflux mediated by SWEET proteins as a key step for phloem transport.
2012,
Pubmed
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1999,
Pubmed
Coruzzi,
Carbon and nitrogen sensing and signaling in plants: emerging 'matrix effects'.
2001,
Pubmed
Coruzzi,
Nitrogen and carbon nutrient and metabolite signaling in plants.
2001,
Pubmed
Czechowski,
Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.
2005,
Pubmed
Dekkers,
Glucose delays seed germination in Arabidopsis thaliana.
2004,
Pubmed
Dekkers,
Interaction between sugar and abscisic acid signalling during early seedling development in Arabidopsis.
2008,
Pubmed
Desbrosses-Fonrouge,
Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation.
2005,
Pubmed
,
Xenbase
Endler,
Identification of a vacuolar sucrose transporter in barley and Arabidopsis mesophyll cells by a tonoplast proteomic approach.
2006,
Pubmed
Eom,
Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth.
2011,
Pubmed
Fettke,
Identification, subcellular localization and biochemical characterization of water-soluble heteroglycans (SHG) in leaves of Arabidopsis thaliana L.: distinct SHG reside in the cytosol and in the apoplast.
2005,
Pubmed
Gerhardt,
Subcellular Metabolite Levels in Spinach Leaves : Regulation of Sucrose Synthesis during Diurnal Alterations in Photosynthetic Partitioning.
2010,
Pubmed
Getz,
Characteristics of Sucrose Transport and Sucrose-Induced H+ Transport on the Tonoplast of Red Beet (Beta vulgaris L.) Storage Tissue.
2002,
Pubmed
Granot,
Hexose kinases and their role in sugar-sensing and plant development.
2013,
Pubmed
Hannah,
A global survey of gene regulation during cold acclimation in Arabidopsis thaliana.
2006,
Pubmed
Hirel,
The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches.
2007,
Pubmed
Jang,
Hexokinase as a sugar sensor in higher plants.
1997,
Pubmed
Kaiser,
Sucrose transport into vacuoles isolated from barley mesophyll protoplasts.
2013,
Pubmed
Karimi,
GATEWAY vectors for Agrobacterium-mediated plant transformation.
2002,
Pubmed
Koch,
CARBOHYDRATE-MODULATED GENE EXPRESSION IN PLANTS.
2004,
Pubmed
Kovermann,
The Arabidopsis vacuolar malate channel is a member of the ALMT family.
2007,
Pubmed
,
Xenbase
Krapp,
Arabidopsis roots and shoots show distinct temporal adaptation patterns toward nitrogen starvation.
2011,
Pubmed
Lalonde,
Transport mechanisms for organic forms of carbon and nitrogen between source and sink.
2004,
Pubmed
Marger,
A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport.
1993,
Pubmed
Martinoia,
Vacuolar transporters and their essential role in plant metabolism.
2006,
Pubmed
Martinoia,
Vacuolar transporters in their physiological context.
2012,
Pubmed
McAllister,
Engineering nitrogen use efficient crop plants: the current status.
2012,
Pubmed
Neuhaus,
Transport of primary metabolites across the plant vacuolar membrane.
2007,
Pubmed
Nägele,
Approximating subcellular organisation of carbohydrate metabolism during cold acclimation in different natural accessions of Arabidopsis thaliana.
2013,
Pubmed
Nägele,
Mathematical modeling of the central carbohydrate metabolism in Arabidopsis reveals a substantial regulatory influence of vacuolar invertase on whole plant carbon metabolism.
2010,
Pubmed
Payyavula,
The sucrose transporter family in Populus: the importance of a tonoplast PtaSUT4 to biomass and carbon partitioning.
2011,
Pubmed
Poschet,
A novel Arabidopsis vacuolar glucose exporter is involved in cellular sugar homeostasis and affects the composition of seed storage compounds.
2011,
Pubmed
Quick,
Short-term water stress leads to a stimulation of sucrose synthesis by activating sucrose-phosphate synthase.
2013,
Pubmed
Reinders,
Functional analysis of LjSUT4, a vacuolar sucrose transporter from Lotus japonicus.
2008,
Pubmed
,
Xenbase
Ristic,
Ultrastructural Evidence That Intracellular Ice Formation and Possibly Cavitation Are the Sources of Freezing Injury in Supercooling Wood Tissue of Cornus florida L.
2002,
Pubmed
Roitsch,
Function and regulation of plant invertases: sweet sensations.
2004,
Pubmed
Rolland,
Sugar sensing and signaling in plants.
2002,
Pubmed
Scheible,
Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen.
2004,
Pubmed
Schneider,
Vacuoles release sucrose via tonoplast-localised SUC4-type transporters.
2012,
Pubmed
Schulz,
Proton-driven sucrose symport and antiport are provided by the vacuolar transporters SUC4 and TMT1/2.
2011,
Pubmed
Schulze,
Cold acclimation induces changes in Arabidopsis tonoplast protein abundance and activity and alters phosphorylation of tonoplast monosaccharide transporters.
2012,
Pubmed
Schumacher,
The V-ATPase: small cargo, large effects.
2010,
Pubmed
Shan,
Comparison of phytohormone signaling mechanisms.
2012,
Pubmed
Smeekens,
Sugar Sensing and Sugar-Mediated Signal Transduction in Plants.
2002,
Pubmed
Szecowka,
Metabolic fluxes in an illuminated Arabidopsis rosette.
2013,
Pubmed
Turgeon,
Phloem transport: cellular pathways and molecular trafficking.
2009,
Pubmed
Willenbrink,
Characteristics of the sucrose uptake system of vacuoles isolated from red beet tissue. Kinetics and specifity of the sucrose uptake system.
2013,
Pubmed
Wingenter,
Increased activity of the vacuolar monosaccharide transporter TMT1 alters cellular sugar partitioning, sugar signaling, and seed yield in Arabidopsis.
2010,
Pubmed
Wingenter,
A member of the mitogen-activated protein 3-kinase family is involved in the regulation of plant vacuolar glucose uptake.
2011,
Pubmed
Wormit,
Molecular identification and physiological characterization of a novel monosaccharide transporter from Arabidopsis involved in vacuolar sugar transport.
2007,
Pubmed
Yamada,
Functional analysis of an Arabidopsis thaliana abiotic stress-inducible facilitated diffusion transporter for monosaccharides.
2010,
Pubmed
Yoo,
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.
2007,
Pubmed
Zuther,
The role of raffinose in the cold acclimation response of Arabidopsis thaliana.
2004,
Pubmed
Zwieniecki,
Confronting Maxwell's demon: biophysics of xylem embolism repair.
2009,
Pubmed
