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Proc Natl Acad Sci U S A
2012 Aug 21;10934:13579-83. doi: 10.1073/pnas.1207606109.
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Structural insights into neuronal K+ channel-calmodulin complexes.
Mruk K
,
Shandilya SM
,
Blaustein RO
,
Schiffer CA
,
Kobertz WR
.
Abstract
Calmodulin (CaM) is a ubiquitous intracellular calcium sensor that directly binds to and modulates a wide variety of ion channels. Despite the large repository of high-resolution structures of CaM bound to peptide fragments derived from ion channels, there is no structural information about CaM bound to a fully folded ion channel at the plasma membrane. To determine the location of CaM docked to a functioning KCNQ K(+) channel, we developed an intracellular tethered blocker approach to measure distances between CaM residues and the ion-conducting pathway. Combining these distance restraints with structural bioinformatics, we generated an archetypal quaternary structural model of an ion channel-CaM complex in the open state. These models place CaM close to the cytoplasmic gate, where it is well positioned to modulate channel function.
Alaimo,
Calmodulin activation limits the rate of KCNQ2 K+ channel exit from the endoplasmic reticulum.
2009, Pubmed,
Xenbase
Alaimo,
Calmodulin activation limits the rate of KCNQ2 K+ channel exit from the endoplasmic reticulum.
2009,
Pubmed
,
Xenbase
Allen,
Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments.
2004,
Pubmed
Bal,
Ca2+/calmodulin disrupts AKAP79/150 interactions with KCNQ (M-Type) K+ channels.
2010,
Pubmed
Black,
Calmodulin interactions with IQ peptides from voltage-dependent calcium channels.
2005,
Pubmed
Blaustein,
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
2000,
Pubmed
,
Xenbase
Cartaud,
Xenopus laevis oocyte calmodulin in the process of meiotic maturation.
1980,
Pubmed
,
Xenbase
Cicirelli,
Internal pH of Xenopus oocytes: a study of the mechanism and role of pH changes during meiotic maturation.
1983,
Pubmed
,
Xenbase
Cicirelli,
Calmodulin synthesis and accumulation during oogenesis and maturation of Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase
Contessa,
Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family.
2005,
Pubmed
,
Xenbase
Cukovic,
A discrete amino terminal domain of Kv1.5 and Kv1.4 potassium channels interacts with the spectrin repeats of alpha-actinin-2.
2001,
Pubmed
DeMaria,
Calmodulin bifurcates the local Ca2+ signal that modulates P/Q-type Ca2+ channels.
2001,
Pubmed
Erickson,
FRET two-hybrid mapping reveals function and location of L-type Ca2+ channel CaM preassociation.
2003,
Pubmed
Etxeberria,
Calmodulin regulates the trafficking of KCNQ2 potassium channels.
2008,
Pubmed
Etzioni,
Regulation of neuronal M-channel gating in an isoform-specific manner: functional interplay between calmodulin and syntaxin 1A.
2011,
Pubmed
,
Xenbase
Fallon,
Crystal structure of dimeric cardiac L-type calcium channel regulatory domains bridged by Ca2+* calmodulins.
2009,
Pubmed
Gamper,
Structural requirements for differential sensitivity of KCNQ K+ channels to modulation by Ca2+/calmodulin.
2005,
Pubmed
Gamper,
Calmodulin mediates Ca2+-dependent modulation of M-type K+ channels.
2003,
Pubmed
Ghosh,
KCNQ1 assembly and function is blocked by long-QT syndrome mutations that disrupt interaction with calmodulin.
2006,
Pubmed
,
Xenbase
Gopalakrishna,
Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography.
1982,
Pubmed
Gómez-Posada,
Kv7 channels can function without constitutive calmodulin tethering.
2011,
Pubmed
Hirschberg,
Gating of recombinant small-conductance Ca-activated K+ channels by calcium.
1998,
Pubmed
,
Xenbase
Holmgren,
The activation gate of a voltage-gated K+ channel can be trapped in the open state by an intersubunit metal bridge.
1998,
Pubmed
Kim,
Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization.
2010,
Pubmed
,
Xenbase
Klevit,
1H-NMR studies of calmodulin. The nature of the Ca2+-dependent conformational change.
1984,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Maylie,
Small conductance Ca2+-activated K+ channels and calmodulin.
2004,
Pubmed
Mori,
Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin: high-resolution mechanistic implications for channel regulation by Ca2+.
2008,
Pubmed
Morin,
Tethering chemistry and K+ channels.
2008,
Pubmed
O'Connell,
Xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.
2011,
Pubmed
,
Xenbase
Regev,
Selective interaction of syntaxin 1A with KCNQ2: possible implications for specific modulation of presynaptic activity.
2009,
Pubmed
,
Xenbase
Rhoads,
Sequence motifs for calmodulin recognition.
1997,
Pubmed
Schumacher,
Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin.
2001,
Pubmed
Schönherr,
Inhibition of human ether à go-go potassium channels by Ca(2+)/calmodulin.
2000,
Pubmed
,
Xenbase
Shamgar,
Calmodulin is essential for cardiac IKS channel gating and assembly: impaired function in long-QT mutations.
2006,
Pubmed
Suh,
Electrostatic interaction of internal Mg2+ with membrane PIP2 Seen with KCNQ K+ channels.
2007,
Pubmed
Thompson,
Affinity and location of an internal K+ ion binding site in shaker K channels.
2001,
Pubmed
,
Xenbase
Wen,
Calmodulin is an auxiliary subunit of KCNQ2/3 potassium channels.
2002,
Pubmed
Wiener,
The KCNQ1 (Kv7.1) COOH terminus, a multitiered scaffold for subunit assembly and protein interaction.
2008,
Pubmed
Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
Yus-Najera,
The identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent KCNQ potassium channels.
2002,
Pubmed
Zhou,
Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.
2001,
Pubmed
,
Xenbase
