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We report the isolation of Xdtx1, a Xenopus homologue of the Drosophila Deltex gene. Starting from tailbud stage, Xdtx1 transcripts are detected in the olfactory bulbs, pineal complex and along the neural tube according to an antero-posterior gradient showing a gap at the midbrain-hindbrain boundary. At tadpole stage, Xdtx1 expression is activated in the differentiating retina, where it is also found in the neuronal fibres of the outer and inner plexiform layers, while its expression in the pineal complex becomes restricted to the photosensitive frontal organ. Differently from other vertebrate Deltex homologues, Xdtx1 is exclusively expressed in regions undergoing neuronal differentiation as shown by complementarity with X-Notch-1 expression.
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Fig. 2. Expression pattern of Xdtx1. The probes used are indicated on the top of each panel. (A–H) Whole-mount in situ hybridizations. (A,B) Stage 26: lateral view, anterior to the left (A) and frontal view, dorsal to the top (B). (C–H) Stage 32: lateral views, anterior to the left (C,F–H), frontal view, dorsal to the top (D) and dorsal view, anterior to the left (E). (I–P) Transversal sections of whole-mount processed embryos at stage 32. Sections from (I) to (M) are progressively ordered along the rostro-caudal axis, as shown in (E), and correspond to telencephalon (I), anteriordiencephalon (J), posteriordiencephalon (K), hindbrain (L) and spinal cord (M). (N–P) Spinal cord sections. (Q–S) Transversal sections of whole-mount processed embryos at stage 40 corresponding to telencephalon (Q), diencephalon (R) and hindbrain (S). hy, hypothalamus. The red arrowheads point to the pineal complex while the black arrowhead indicates Xpax2 expression at the midbrain–hindbrain boundary.
Fig. 3. Expression of Xdtx1 in the eye and in the frontal organ. Transversal sections of whole-mount processed embryos at stage 32 (A,D) and stage 40 (B,C,E–G), cut at the level of the eyes (A–E) and the frontal organ (F,G). The boxed area in (B) is shown in (C) at higher magnification. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.
dtx1 (deltex 1) gene expression in X, laevis at NF stage 32, assayed via ISH, lateral view, anteriorleft, dorsal up.
