Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Biol 2013 Jan 01;3731:39-52. doi: 10.1016/j.ydbio.2012.10.003.
Show Gene links Show Anatomy links

The Xenopus doublesex-related gene Dmrt5 is required for olfactory placode neurogenesis.

Parlier D , Moers V , Van Campenhout C , Preillon J , Leclère L , Saulnier A , Sirakov M , Busengdal H , Kricha S , Marine JC , Rentzsch F , Bellefroid EJ .

The Dmrt (doublesex and mab-3 related transcription factor) genes encode a large family of evolutionarily conserved transcription factors whose function in sex specific differentiation has been well studied in all animal lineages. In vertebrates, their function is not restricted to the developing gonads. For example, Xenopus Dmrt4 is essential for neurogenesis in the olfactory system. Here we have isolated and characterized Xenopus Dmrt5 and found that it is coexpressed with Dmrt4 in the developing olfactory placodes. As Dmrt4, Dmrt5 is positively regulated in the ectoderm by neural inducers and negatively by proneural factors. Both Dmrt5 and Dmrt4 genes are also activated by the combined action of the transcription factor Otx2, broadly transcribed in the head ectoderm and of Notch signaling, activated in the anterior neural ridge. As for Dmrt4, knockdown of Dmrt5 impairs neurogenesis in the embryonic olfactory system and in neuralized animal caps. Conversely, its overexpression promotes neuronal differentiation in animal caps, a property that requires the conserved C-terminal DMA and DMB domains. We also found that the sea anenome Dmrt4/5 related gene NvDmrtb also induces neurogenesis in Xenopus animal caps and that conversely, its knockdown in Nematostella reduces elav-1 positive neurons. Together, our data identify Dmrt5 as a novel important regulator of neurogenesis whose function overlaps with that of Dmrt4 during Xenopus olfactory system development. They also suggest that Dmrt may have had a role in neurogenesis in the last common ancestor of cnidarians and bilaterians.

PubMed ID: 23064029
Article link: Dev Biol

Species referenced: Xenopus
Genes referenced: ag1 atoh7 dll1 dlx6 dmrta1 dmrta2 ebf2 ebf3 foxe3 foxg1 gal.1 krt12.4 mhc2-dma mhc2-dmb myc neurog2 nkx2-1 nog notch1 otx2 runx1 snai2 sox2 tubb2b
GO keywords: neurogenesis [+]
Antibodies: Myc Ab2
Morpholinos: dmrta1 MO1 dmrta2 MO1

Article Images: [+] show captions