Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract??? Pancreas-specific transcription factor 1a (Ptf1a), a bHLH transcription factor, has two temporally distinct functions during pancreas development; initially it is required for early specification of the entire pancreas, while later it is required for proper differentiation and maintenance of only acinar cells. The importance of Ptf1a function was revealed by the fact that loss of Ptf1a leads to pancreas agenesis in humans. While Ptf1a is one of the most important pancreatic transcription factors, little is known about the differences between the regulatory networks it controls during initial specification of the pancreas as opposed to acinar cell development, and to date no comprehensive analysis of its downstream targets has been published. In this article, we use Xenopus embryos to identify putative downstream targets of Ptf1a. We isolated anterior endodermtissue overexpressing Ptf1a at two early stages, NF32 and NF36, and compared their gene expression profiles using microarrays. Our results revealed that Ptf1a regulates genes with a wide variety of functions, providing insight into the complexity of the regulatory network required for pancreas specification.
Figure 2. RT-PCR verification of selected differentially expressed genes from the microarray analyses. GFP and Ptf1a sample data provided from three different cycle time-points. GFP, gfp mRNA-injected control; Ptf1a, ptf1a mRNA-injected; -RT, control reaction without reverse transcriptase. Reproduced with permission of the Publisher, John Wiley & Sons.
irf1 (interferon regulatory factor 1 ) gene expression in dissected Xenopus laevis gut , assayed via in situ hybridization, NF stage 44.
stxbp1 (syntaxin binding protein 1 ) gene expression in dissected Xenopus laevis gut, assayed via in situ hybridization, NF stage 44.
stx1b (syntaxin 1B ) gene expression in dissected Xenopus laevis gut, assayed via in situ hybridization, NF stage 44.
Afelik,
Combined ectopic expression of Pdx1 and Ptf1a/p48 results in the stable conversion of posterior endoderm into endocrine and exocrine pancreatic tissue.
2006, Pubmed,
Xenbase
Afelik,
Combined ectopic expression of Pdx1 and Ptf1a/p48 results in the stable conversion of posterior endoderm into endocrine and exocrine pancreatic tissue.
2006,
Pubmed
,
Xenbase
Bilogan,
Xenopus staufen2 is required for anterior endodermal organ formation.
2012,
Pubmed
,
Xenbase
Blitz,
Germ layers to organs: using Xenopus to study "later" development.
2006,
Pubmed
,
Xenbase
Cavaillé,
IGFBP-1 inhibits EGF mitogenic activity in cultured endometrial stromal cells.
2006,
Pubmed
Chakraborty,
Regulation of human trophoblast migration and invasiveness.
2002,
Pubmed
Comper,
Physiological function of connective tissue polysaccharides.
1978,
Pubmed
Dong,
Graded levels of Ptf1a differentially regulate endocrine and exocrine fates in the developing pancreas.
2008,
Pubmed
Duan,
Specifying the cellular responses to IGF signals: roles of IGF-binding proteins.
2002,
Pubmed
Edgar,
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository.
2002,
Pubmed
Esni,
Notch inhibits Ptf1 function and acinar cell differentiation in developing mouse and zebrafish pancreas.
2004,
Pubmed
Ferro-Novick,
Vesicle fusion from yeast to man.
1994,
Pubmed
Fujita,
Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements.
1988,
Pubmed
Ghosh,
Interactions between hairy/enhancer of split-related proteins and the pancreatic transcription factor Ptf1-p48 modulate function of the PTF1 transcriptional complex.
2006,
Pubmed
Gittes,
Developmental biology of the pancreas: a comprehensive review.
2009,
Pubmed
Gockerman,
Insulin-like growth factor (IGF)-binding proteins inhibit the smooth muscle cell migration responses to IGF-I and IGF-II.
1995,
Pubmed
Guilbault,
Distinct pattern of lung gene expression in the Cftr-KO mice developing spontaneous lung disease compared with their littermate controls.
2006,
Pubmed
Harland,
Xenopus research: metamorphosed by genetics and genomics.
2011,
Pubmed
,
Xenbase
Hata,
Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin.
1993,
Pubmed
Horb,
Expression of amylase and other pancreatic genes in Xenopus.
2002,
Pubmed
,
Xenbase
Horb,
Xenopus insm1 is essential for gastrointestinal and pancreatic endocrine cell development.
2009,
Pubmed
,
Xenbase
Horb,
BrunoL1 regulates endoderm proliferation through translational enhancement of cyclin A2 mRNA.
2010,
Pubmed
,
Xenbase
Horb,
Experimental conversion of liver to pancreas.
2003,
Pubmed
,
Xenbase
Hosono,
The unc-18 gene encodes a novel protein affecting the kinetics of acetylcholine metabolism in the nematode Caenorhabditis elegans.
1992,
Pubmed
Huang,
Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists.
2009,
Pubmed
Huang,
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
2009,
Pubmed
Hwa,
The insulin-like growth factor-binding protein (IGFBP) superfamily.
1999,
Pubmed
Ito,
XHAPLN3 plays a key role in cardiogenesis by maintaining the hyaluronan matrix around heart anlage.
2008,
Pubmed
,
Xenbase
Jacobsson,
Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells.
1994,
Pubmed
Jarikji,
The tetraspanin Tm4sf3 is localized to the ventral pancreas and regulates fusion of the dorsal and ventral pancreatic buds.
2009,
Pubmed
,
Xenbase
Jarikji,
Differential ability of Ptf1a and Ptf1a-VP16 to convert stomach, duodenum and liver to pancreas.
2007,
Pubmed
,
Xenbase
Jeon,
Differentially up-regulated genes in proliferating porcine neonatal pancreas cells caused by epidermal growth factor.
2004,
Pubmed
Johansson,
Lack of nuclear expression of hairy and enhancer of split-1 (HES1) in pancreatic endocrine tumors.
2008,
Pubmed
Jones,
Insulin-like growth factor binding protein 1 stimulates cell migration and binds to the alpha 5 beta 1 integrin by means of its Arg-Gly-Asp sequence.
1993,
Pubmed
Kawaguchi,
The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors.
2002,
Pubmed
Kelly,
Development of the pancreas in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Krapp,
The p48 DNA-binding subunit of transcription factor PTF1 is a new exocrine pancreas-specific basic helix-loop-helix protein.
1996,
Pubmed
Krapp,
The bHLH protein PTF1-p48 is essential for the formation of the exocrine and the correct spatial organization of the endocrine pancreas.
1998,
Pubmed
Kröger,
Activities of IRF-1.
2002,
Pubmed
Lee,
Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein.
1995,
Pubmed
,
Xenbase
Lehtonen,
Vesicular transport and kidney development.
1999,
Pubmed
Lin,
Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas.
2004,
Pubmed
Masui,
Early pancreatic development requires the vertebrate Suppressor of Hairless (RBPJ) in the PTF1 bHLH complex.
2007,
Pubmed
Miller,
IDX-1: a new homeodomain transcription factor expressed in rat pancreatic islets and duodenum that transactivates the somatostatin gene.
1994,
Pubmed
,
Xenbase
Novak,
Generalization of DNA microarray dispersion properties: microarray equivalent of t-distribution.
2006,
Pubmed
Novak,
Characterization of variability in large-scale gene expression data: implications for study design.
2002,
Pubmed
Novak,
Variation in fiberoptic bead-based oligonucleotide microarrays: dispersion characteristics among hybridization and biological replicate samples.
2006,
Pubmed
Oh,
Munc18c depletion selectively impairs the sustained phase of insulin release.
2009,
Pubmed
Oropeza,
Transient expression of Ngn3 in Xenopus endoderm promotes early and ectopic development of pancreatic beta and delta cells.
2012,
Pubmed
,
Xenbase
Pearl,
Functional analysis of Rfx6 and mutant variants associated with neonatal diabetes.
2011,
Pubmed
,
Xenbase
Pearl,
Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center.
2012,
Pubmed
,
Xenbase
Pearl,
Xenopus pancreas development.
2009,
Pubmed
,
Xenbase
Sellick,
Mutations in PTF1A cause pancreatic and cerebellar agenesis.
2004,
Pubmed
Shimabukuro,
Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells.
2005,
Pubmed
Sommer,
neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS.
1996,
Pubmed
,
Xenbase
Spurlin,
Syntaxin 4 transgenic mice exhibit enhanced insulin-mediated glucose uptake in skeletal muscle.
2004,
Pubmed
Tomas,
Munc 18-1 and granuphilin collaborate during insulin granule exocytosis.
2008,
Pubmed
Whiteford,
Structural and cell adhesion properties of zebrafish syndecan-4 are shared with higher vertebrates.
2008,
Pubmed
Zecchin,
Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates.
2004,
Pubmed
Zhang,
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell.
2000,
Pubmed
