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Biochem J
2012 Aug 15;4461:47-58. doi: 10.1042/BJ20120467.
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Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice.
Guzeloglu-Kayisli O
,
Lalioti MD
,
Aydiner F
,
Sasson I
,
Ilbay O
,
Sakkas D
,
Lowther KM
,
Mehlmann LM
,
Seli E
.
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Gene expression during oocyte maturation and early embryogenesis up to zygotic genome activation requires translational activation of maternally-derived mRNAs. EPAB [embryonic poly(A)-binding protein] is the predominant poly(A)-binding protein during this period in Xenopus, mouse and human. In Xenopus oocytes, ePAB stabilizes maternal mRNAs and promotes their translation. To assess the role of EPAB in mammalian reproduction, we generated Epab-knockout mice. Although Epab(-/-) males and Epab(+/-) of both sexes were fertile, Epab(-/-) female mice were infertile, and could not generate embryos or mature oocytes in vivo or in vitro. Epab(-/-) oocytes failed to achieve translational activation of maternally-stored mRNAs upon stimulation of oocyte maturation, including Ccnb1 (cyclin B1) and Dazl (deleted in azoospermia-like) mRNAs. Microinjection of Epab mRNA into Epab(-/-) germinal vesicle stage oocytes did not rescue maturation, suggesting that EPAB is also required for earlier stages of oogenesis. In addition, late antral follicles in the ovaries of Epab(-/-) mice exhibited impaired cumulus expansion, and a 8-fold decrease in ovulation, associated with a significant down-regulation of mRNAs encoding the EGF (epidermal growth factor)-like growth factors Areg (amphiregulin), Ereg (epiregulin) and Btc (betacellulin), and their downstream regulators, Ptgs2 (prostaglandin synthase 2), Has2 (hyaluronan synthase 2) and Tnfaip6 (tumour necrosis factor α-induced protein 6). The findings from the present study indicate that EPAB is necessary for oogenesis, folliculogenesis and female fertility in mice.
Adashi,
Endocrinology of the ovary.
1994,
Pubmed
Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase
Chen,
Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition.
2011,
Pubmed
,
Xenbase
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase
Davis,
Anovulation in cyclooxygenase-2-deficient mice is restored by prostaglandin E2 and interleukin-1beta.
1999,
Pubmed
Diaz,
Oocytes determine cumulus cell lineage in mouse ovarian follicles.
2007,
Pubmed
Dinchuk,
Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II.
1995,
Pubmed
Eppig,
Intercommunication between mammalian oocytes and companion somatic cells.
1991,
Pubmed
Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase
Flach,
The transition from maternal to embryonic control in the 2-cell mouse embryo.
1982,
Pubmed
Freimann,
EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins/cAMP in human ovarian follicular cells.
2004,
Pubmed
Friend,
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
2012,
Pubmed
,
Xenbase
Fülöp,
Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice.
2003,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed
Gebauer,
Mouse cytoplasmic polyadenylylation element binding protein: an evolutionarily conserved protein that interacts with the cytoplasmic polyadenylylation elements of c-mos mRNA.
1996,
Pubmed
,
Xenbase
Goldman,
The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies.
2007,
Pubmed
Guzeloglu-Kayisli,
Identification and characterization of human embryonic poly(A) binding protein (EPAB).
2008,
Pubmed
,
Xenbase
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Hampl,
Translational regulation of the gradual increase in histone H1 kinase activity in maturing mouse oocytes.
1995,
Pubmed
Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase
Hsieh,
Epidermal growth factor-like growth factors in the follicular fluid: role in oocyte development and maturation.
2009,
Pubmed
Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed
Kim,
RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB.
2007,
Pubmed
,
Xenbase
Kline,
Quantitative microinjection of mouse oocytes and eggs.
2009,
Pubmed
Lin,
Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice.
2005,
Pubmed
Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed
Masciarelli,
Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of female infertility.
2004,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Matova,
Comparative aspects of animal oogenesis.
2001,
Pubmed
,
Xenbase
Mehlmann,
The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes.
2004,
Pubmed
Mueller,
Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15.
1995,
Pubmed
,
Xenbase
Nakanishi,
Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation.
2006,
Pubmed
,
Xenbase
Ochsner,
Disrupted function of tumor necrosis factor-alpha-stimulated gene 6 blocks cumulus cell-oocyte complex expansion.
2003,
Pubmed
Ozturk,
Epab and Pabpc1 are differentially expressed during male germ cell development.
2012,
Pubmed
Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase
Park,
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
2004,
Pubmed
Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed
Pasparakis,
TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2.
2002,
Pubmed
Piccioni,
Translational regulation during oogenesis and early development: the cap-poly(A) tail relationship.
2005,
Pubmed
Polanski,
Cyclin synthesis controls the progression of meiotic maturation in mouse oocytes.
1998,
Pubmed
Racki,
CPEB controls oocyte growth and follicle development in the mouse.
2006,
Pubmed
Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Richards,
Ovulation: new dimensions and new regulators of the inflammatory-like response.
2002,
Pubmed
Ruggiu,
The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis.
1997,
Pubmed
Sallés,
Rapid and sensitive analysis of mRNA polyadenylation states by PCR.
1995,
Pubmed
,
Xenbase
Sauer,
Inducible gene targeting in mice using the Cre/lox system.
1998,
Pubmed
Sekiguchi,
Expression of epiregulin and amphiregulin in the rat ovary.
2004,
Pubmed
Seli,
An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos.
2005,
Pubmed
,
Xenbase
Shimada,
Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor.
2006,
Pubmed
Solomon,
CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15.
1993,
Pubmed
,
Xenbase
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Tay,
The control of cyclin B1 mRNA translation during mouse oocyte maturation.
2000,
Pubmed
Tay,
Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.
2001,
Pubmed
Vanderhyden,
Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation.
1990,
Pubmed
Vasudevan,
Metazoan oocyte and early embryo development program: a progression through translation regulatory cascades.
2006,
Pubmed
,
Xenbase
Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Wassarman,
Meiotic maturation of mouse oocytes in vitro: inhibition of maturation at specific stages of nuclear progression.
1976,
Pubmed
Wells,
The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression.
1999,
Pubmed
White,
The nuclear receptor co-repressor nrip1 (RIP140) is essential for female fertility.
2000,
Pubmed
Wilkie,
Embryonic poly(A)-binding protein stimulates translation in germ cells.
2005,
Pubmed
,
Xenbase
Winston,
Stability of cyclin B protein during meiotic maturation and the first mitotic cell division in mouse oocytes.
1997,
Pubmed
de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed
