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Genesis 2012 Mar 01;503:325-32. doi: 10.1002/dvg.22006.
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Site-specific transgenesis in Xenopus.

Zuber ME , Nihart HS , Zhuo X , Babu S , Knox BE .

Transgenesis is an essential, powerful tool for investigating gene function and the activities of enhancers, promoters, and transcription factors in the chromatin environment. In Xenopus, current methods generate germ-line transgenics by random insertion, often resulting in mosaicism, position-dependent variations in expression, and lab-to-lab differences in efficiency. We have developed and tested a Xenopus FLP-FRT recombinase-mediated transgenesis (X-FRMT) method. We demonstrate transgenesis of Xenopus laevis by FLP-catalyzed recombination of donor plasmid cassettes into F(1) tadpoles with host cassette transgenes. X-FRMT provides a new method for generating transgenic Xenopus. Once Xenopus lines harboring single host cassettes are generated, X-FRMT should allow for the targeting of transgenes to well-characterized integration site(s), requiring no more special reagents or training than that already common to most Xenopus labs.

PubMed ID: 22337567
PMC ID: PMC3294184
Article link: Genesis
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a six6 tbxt.2

Article Images: [+] show captions
References [+] :
Buchholz, Improved properties of FLP recombinase evolved by cycling mutagenesis. 1998, Pubmed