Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Dyn 2011 Jun 01;2406:1430-9. doi: 10.1002/dvdy.22643.
Show Gene links Show Anatomy links

Rab3d is required for Xenopus anterior neurulation by regulating Noggin secretion.

Rab3d is a member of the Ras-related small GTPase family of secretory Rab, Rab3. In this study, we showed that Xenopus Rab3d is expressed specifically in the anterior border of the neural plate when the neural plate converges and folds to initiate neural tube formation. Morpholino-mediated knockdown of Rab3d resulted in neurulation defects both in neural plate convergence and folding. Interestingly, perturbation of BMP signaling rescued neurulation defects of Rab3d morphants, suggesting that Rab3d inhibits BMP signaling during neurulation. By secretion assay in the Xenopus animal cap, we found that Rab3d specifically regulates secretion of a BMP antagonist, Noggin, but not Chordin and Wnts. We also found that Rab3d is co-localized with Noggin and that this interaction is dependent on the GTP/GDP cycle of Rab3d. Collectively, these findings suggest that Rab3d-mediated secretion regulation of a BMP antagonist, Noggin, is one of the mechanisms of BMP antagonism during Xenopus anterior neurulation.

PubMed ID: 21520330
Article link: Dev Dyn

Species referenced: Xenopus laevis
Genes referenced: actl6a chrd.1 egr2 msx1 myc nog otx2 rab3d smad1 sox2 tubb2b wnt5a wnt8a
Antibodies: Smad1 Ab8
Morpholinos: rab3d MO1

Article Images: [+] show captions