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Dev Biol 2011 Jan 15;3492:204-12. doi: 10.1016/j.ydbio.2010.10.015.
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The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis.

Abu-Daya A , Nishimoto S , Fairclough L , Mohun TJ , Logan MP , Zimmerman LB .

While limb regeneration has been extensively studied in amphibians, little is known about the initial events in limb formation in metamorphosing anurans. The small secreted integrin ligand nephronectin (npnt) is necessary for development of the metanephros in mouse. Although expressed in many tissues, its role in other developmental processes is not well-studied. Here we show that a transgene insertion that disrupts this gene ablates forelimb formation in Xenopus tropicalis. Our results suggest a novel role for integrin signalling in limb development, and represent the first insertional phenotype to be cloned in amphibians.

PubMed ID: 20977901
PMC ID: PMC3021715
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: egf itga8 itgb1 npnt odc1 pax8 tbx5
Morpholinos: npnt MO1 npnt MO2 npnt MO3

Phenotypes: Xtr.npnt{insZimml} + npnt MO (Fig 4. A, B) [+]

Article Images: [+] show captions
References [+] :
Agarwal, Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. 2003, Pubmed