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Dev Dyn
2010 Oct 01;23910:2627-36. doi: 10.1002/dvdy.22399.
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Zebrafish cx30.3: identification and characterization of a gap junction gene highly expressed in the skin.
Tao L
,
DeRosa AM
,
White TW
,
Valdimarsson G
.
Abstract
We have identified and characterized a zebrafish connexin, Cx30.3. Sequence similarity analyses suggested that Cx30.3 was orthologous to both mammalian Cx26 and Cx30, known to play important roles in the skin and inner ear of mammals. Analysis of mRNA expression showed that Cx30.3 was present in early embryos, and was highly abundant in skin, but also detected in other tissues including fins, inner ear, heart, and the retina. Injection of Cx30.3 cRNA into Xenopus oocytes elicited robust intercellular coupling with voltage gating sensitivity similar to mammalian Cx26 and Cx30. The similarities in functional properties and expression patterns suggest that Cx30.3, like mammalian Cx26 and Cx30, may play a significant role in skin development, hearing, and balance in zebrafish. Thus, zebrafish could potentially serve as an excellent model to study disorders of the skin and deafness that result from human connexin mutations.
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Beyer,
Connexin family of gap junction proteins.
1990,
Pubmed
Bruzzone,
The cellular Internet: on-line with connexins.
1996,
Pubmed
Cason,
Molecular cloning, expression analysis, and functional characterization of connexin44.1: a zebrafish lens gap junction protein.
2001,
Pubmed
Cheng,
Connexin 48.5 is required for normal cardiovascular function and lens development in zebrafish embryos.
2004,
Pubmed
,
Xenbase
Christie,
Molecular cloning, functional analysis, and RNA expression analysis of connexin45.6: a zebrafish cardiovascular connexin.
2004,
Pubmed
,
Xenbase
Cruciani,
The vertebrate connexin family.
2006,
Pubmed
Cruciani,
Evolutionary selection pressure and family relationships among connexin genes.
2007,
Pubmed
,
Xenbase
Cserzö,
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method.
1997,
Pubmed
Dahl,
Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
1987,
Pubmed
,
Xenbase
Dahl,
Mutational analysis of gap junction formation.
1992,
Pubmed
,
Xenbase
Dahl,
Molecular cloning and functional expression of mouse connexin-30,a gap junction gene highly expressed in adult brain and skin.
1996,
Pubmed
,
Xenbase
DeRosa,
The cataract-inducing S50P mutation in Cx50 dominantly alters the channel gating of wild-type lens connexins.
2007,
Pubmed
,
Xenbase
Dobrowolski,
Connexin-caused genetic diseases and corresponding mouse models.
2009,
Pubmed
Eastman,
Phylogenetic analysis of three complete gap junction gene families reveals lineage-specific duplications and highly supported gene classes.
2006,
Pubmed
Gasteiger,
ExPASy: The proteomics server for in-depth protein knowledge and analysis.
2003,
Pubmed
Gerido,
Connexin disorders of the ear, skin, and lens.
2004,
Pubmed
Ghysen,
Development of the zebrafish lateral line.
2004,
Pubmed
Goodenough,
Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin.
1974,
Pubmed
Goodrich,
Hear, hear for the zebrafish.
2005,
Pubmed
Hasson,
Molecular motors: sensing a function for myosin-VIIa.
1999,
Pubmed
John,
Connexon integrity is maintained by non-covalent bonds: intramolecular disulfide bonds link the extracellular domains in rat connexin-43.
1991,
Pubmed
Kikuchi,
Gap junction systems in the mammalian cochlea.
2000,
Pubmed
Larkin,
Clustal W and Clustal X version 2.0.
2007,
Pubmed
Lefebvre,
Connexins, hearing and deafness: clinical aspects of mutations in the connexin 26 gene.
2000,
Pubmed
Liu,
Mapping the 5' and 3' ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE).
1993,
Pubmed
McLachlan,
Zebrafish Cx35: cloning and characterization of a gap junction gene highly expressed in the retina.
2003,
Pubmed
,
Xenbase
Meşe,
Altered gating properties of functional Cx26 mutants associated with recessive non-syndromic hearing loss.
2004,
Pubmed
,
Xenbase
Meşe,
Gap junctions: basic structure and function.
2007,
Pubmed
Nag,
The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity.
2006,
Pubmed
Oyamada,
Regulation of connexin expression.
2005,
Pubmed
Page,
TreeView: an application to display phylogenetic trees on personal computers.
1996,
Pubmed
Rahman,
Topography of connexin32 in rat liver gap junctions. Evidence for an intramolecular disulphide linkage connecting the two extracellular peptide loops.
1991,
Pubmed
Richard,
Connexins: a connection with the skin.
2000,
Pubmed
Richard,
Connexin disorders of the skin.
2005,
Pubmed
Schaefer,
Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends.
1995,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Söhl,
Gap junctions and the connexin protein family.
2004,
Pubmed
,
Xenbase
Tang,
Validation of zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization.
2007,
Pubmed
Thisse,
Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos.
1993,
Pubmed
Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase
Unger,
Three-dimensional structure of a recombinant gap junction membrane channel.
1999,
Pubmed
White,
Unique and redundant connexin contributions to lens development.
2002,
Pubmed
White,
Nonredundant gap junction functions.
2003,
Pubmed
White,
Genetic diseases and gene knockouts reveal diverse connexin functions.
1999,
Pubmed
Whitfield,
Zebrafish as a model for hearing and deafness.
2002,
Pubmed
