Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Telomerase trafficking and assembly in Xenopus oocytes.
Li ZH
,
Tomlinson RL
,
Terns RM
,
Terns MP
.
???displayArticle.abstract???
The core components of telomerase are telomerase RNA (TR) and telomerase reverse transcriptase (TERT). In vertebrate cells, TR and TERT have been reported to associate with intranuclear structures, including Cajal bodies and nucleoli as well as telomeres. Here, we examined the time course of both TR localization and assembly of TR with TERT in Xenopus oocytes. The major trafficking pathway for microinjected TR is through Cajal bodies into the nucleoplasm, with a fraction of TR found in nucleoli at later time points. Telomerase assembly precedes nucleolar localization of TR, and TR mutants that do not localize to nucleoli form active enzyme, indicating that localization of TR to nucleoli is not required for assembly with TERT. Assembly of telomerase coincides with Cajal-body localization; however, assembly is also unaffected by a CAB-box mutation (which significantly reduces association with Cajal bodies), suggesting that Cajal-body localization is not important for assembly. Our results suggest that assembly of TR with TERT occurs in the nucleoplasm. Unexpectedly, however, our experiments reveal that disruption of the CAB box does not eliminate early targeting to Cajal bodies, indicating that a role for Cajal bodies in telomerase assembly cannot be excluded on the basis of existing knowledge.
Aubert,
Telomeres and aging.
2008,
Pubmed
Autexier,
Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA.
1996,
Pubmed
Autexier,
The structure and function of telomerase reverse transcriptase.
2006,
Pubmed
Beattie,
Reconstitution of human telomerase activity in vitro.
1998,
Pubmed
Blasco,
Telomere length, stem cells and aging.
2007,
Pubmed
Boisvert,
The multifunctional nucleolus.
2007,
Pubmed
Carmo-Fonseca,
New clues to the function of the Cajal body.
2002,
Pubmed
Chen,
Secondary structure of vertebrate telomerase RNA.
2000,
Pubmed
Cohen,
Protein composition of catalytically active human telomerase from immortal cells.
2007,
Pubmed
Collins,
The biogenesis and regulation of telomerase holoenzymes.
2006,
Pubmed
Collins,
Ciliate telomerase biochemistry.
1999,
Pubmed
Cristofari,
Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere elongation.
2007,
Pubmed
Dandjinou,
A phylogenetically based secondary structure for the yeast telomerase RNA.
2004,
Pubmed
Darzacq,
Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs.
2002,
Pubmed
,
Xenbase
Dragon,
In vitro assembly of human H/ACA small nucleolar RNPs reveals unique features of U17 and telomerase RNAs.
2000,
Pubmed
Etheridge,
The nucleolar localization domain of the catalytic subunit of human telomerase.
2002,
Pubmed
Feng,
The RNA component of human telomerase.
1995,
Pubmed
Fu,
Distinct biogenesis pathways for human telomerase RNA and H/ACA small nucleolar RNAs.
2003,
Pubmed
Fu,
Human telomerase and Cajal body ribonucleoproteins share a unique specificity of Sm protein association.
2006,
Pubmed
Gall,
Structure in the amphibian germinal vesicle.
2004,
Pubmed
,
Xenbase
Gall,
The centennial of the Cajal body.
2003,
Pubmed
Gall,
Cajal bodies: the first 100 years.
2000,
Pubmed
Garcia,
Human diseases of telomerase dysfunction: insights into tissue aging.
2007,
Pubmed
Greider,
Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.
1985,
Pubmed
Harley,
Telomerase and cancer therapeutics.
2008,
Pubmed
Jády,
Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body-specific localization signal.
2004,
Pubmed
Jády,
Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres.
2006,
Pubmed
Jády,
Modification of Sm small nuclear RNAs occurs in the nucleoplasmic Cajal body following import from the cytoplasm.
2003,
Pubmed
Kaiser,
De novo formation of a subnuclear body.
2008,
Pubmed
Lange,
Box H and box ACA are nucleolar localization elements of U17 small nucleolar RNA.
1999,
Pubmed
,
Xenbase
Leung,
The dynamics of the nucleolus.
2003,
Pubmed
Lukowiak,
The snoRNA domain of vertebrate telomerase RNA functions to localize the RNA within the nucleus.
2001,
Pubmed
,
Xenbase
Masutomi,
Telomerase activity reconstituted in vitro with purified human telomerase reverse transcriptase and human telomerase RNA component.
2000,
Pubmed
Matera,
Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs.
2007,
Pubmed
Meyerson,
hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization.
1997,
Pubmed
Mitchell,
A box H/ACA small nucleolar RNA-like domain at the human telomerase RNA 3' end.
1999,
Pubmed
Mitchell,
A telomerase component is defective in the human disease dyskeratosis congenita.
1999,
Pubmed
Mitchell,
Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse transcriptase.
2000,
Pubmed
Morris,
The Cajal body.
2008,
Pubmed
Moyzis,
A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
1988,
Pubmed
Nakamura,
Telomerase catalytic subunit homologs from fission yeast and human.
1997,
Pubmed
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Narayanan,
Nucleolar localization signals of box H/ACA small nucleolar RNAs.
1999,
Pubmed
,
Xenbase
Paushkin,
The SMN complex, an assemblyosome of ribonucleoproteins.
2002,
Pubmed
Pederson,
In search of nonribosomal nucleolar protein function and regulation.
2009,
Pubmed
Pogacić,
Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10.
2000,
Pubmed
Seto,
Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle.
1999,
Pubmed
Shay,
Targeting telomerase for cancer therapeutics.
2008,
Pubmed
Shay,
Telomerase: a target for cancer therapeutics.
2002,
Pubmed
Teixeira,
Intracellular trafficking of yeast telomerase components.
2002,
Pubmed
Terns,
Nuclear transport of RNAs in microinjected Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Terns,
Noncoding RNAs of the H/ACA family.
2006,
Pubmed
Terns,
Macromolecular complexes: SMN--the master assembler.
2001,
Pubmed
Tesmer,
Two inactive fragments of the integral RNA cooperate to assemble active telomerase with the human protein catalytic subunit (hTERT) in vitro.
1999,
Pubmed
Theimer,
Structure and function of telomerase RNA.
2006,
Pubmed
Theimer,
Structural and functional characterization of human telomerase RNA processing and cajal body localization signals.
2007,
Pubmed
Tomlinson,
Cell cycle-regulated trafficking of human telomerase to telomeres.
2006,
Pubmed
Tomlinson,
Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells.
2008,
Pubmed
Tycowski,
A conserved WD40 protein binds the Cajal body localization signal of scaRNP particles.
2009,
Pubmed
Tzfati,
A novel pseudoknot element is essential for the action of a yeast telomerase.
2003,
Pubmed
Venteicher,
A human telomerase holoenzyme protein required for Cajal body localization and telomere synthesis.
2009,
Pubmed
Verheggen,
Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments.
2002,
Pubmed
,
Xenbase
Weinrich,
Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT.
1997,
Pubmed
Whitehead,
Determinants of the interaction of the spinal muscular atrophy disease protein SMN with the dimethylarginine-modified box H/ACA small nucleolar ribonucleoprotein GAR1.
2002,
Pubmed
Wong,
Subnuclear shuttling of human telomerase induced by transformation and DNA damage.
2002,
Pubmed
Wright,
Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity.
1995,
Pubmed
Yang,
Nucleolar localization of hTERT protein is associated with telomerase function.
2002,
Pubmed
Zhu,
Telomerase RNA accumulates in Cajal bodies in human cancer cells.
2004,
Pubmed
