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XB-ART-40986
Mol Biol Cell 2010 Mar 15;216:905-13. doi: 10.1091/mbc.e09-11-0974.
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Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail.

Ohe M , Kawamura Y , Ueno H , Inoue D , Kanemori Y , Senoo C , Isoda M , Nakajo N , Sagata N .


Abstract
Emi2 (also called Erp1) inhibits the anaphase-promoting complex/cyclosome (APC/C) and thereby causes metaphase II arrest in unfertilized vertebrate eggs. Both the D-box and the zinc-binding region (ZBR) of Emi2 have been implicated in APC/C inhibition. However, it is not well known how Emi2 interacts with and hence inhibits the APC/C. Here we show that Emi2 binds the APC/C via the C-terminal tail, termed here the RL tail. When expressed in Xenopus oocytes and egg extracts, Emi2 lacking the RL tail fails to interact with and inhibit the APC/C. The RL tail itself can directly bind to the APC/C, and, when added to egg extracts, either an excess of RL tail peptides or anti-RL tail peptide antibody can dissociate endogenous Emi2 from the APC/C, thus allowing APC/C activation. Furthermore, and importantly, the RL tail-mediated binding apparently promotes the inhibitory interactions of the D-box and the ZBR (of Emi2) with the APC/C. Finally, Emi1, a somatic paralog of Emi2, also has a functionally similar RL tail. We propose that the RL tail of Emi1/Emi2 serves as a docking site for the APC/C, thereby promoting the interaction and inhibition of the APC/C by the D-box and the ZBR.

PubMed ID: 20089832
PMC ID: PMC2836971
Article link: Mol Biol Cell


Species referenced: Xenopus laevis
Genes referenced: camk2g cdc20 cdc23 cdc27 fbxo43 fbxo5 gmnn myc plk1 tbx2
Morpholinos: fbxo43 MO1


Article Images: [+] show captions
References [+] :
Castro, The anaphase-promoting complex: a key factor in the regulation of cell cycle. 2005, Pubmed