Wu J et al. (2010), Differential expression of the Brunol/CELF fami...

XB-ART-40371

Int J Dev Biol 2010 Jan 01;541:209-14. doi: 10.1387/ijdb.082685jw.

Show Gene links Show Anatomy links

Differential expression of the Brunol/CELF family genes during Xenopus laevis early development.

Wu J , Li C , Zhao S , Mao B .

???displayArticle.abstract???
The BRUNOL/CELF family of RNA-binding proteins plays important roles in post-transcriptional regulation and has been implicated in several developmental processes. In this study, we describe the cloning and expression patterns of five Brunol genes in Xenopus laevis. Among them, only Brunol2 is maternally expressed and the zygotic expression of the other four Brunol genes starts at different developmental stages. During Xenopus development, Brunol1, 4-5 are exclusively expressed in the nervous system including domains in the brain, spinal cord, optic and otic vesicles. Brunol2 and 3 are expressed in both the somatic mesoderm and the nervous system. Brunol2 is also extensively expressed in the lens. In transfected Hela cells, BRUNOL1, 2 and 3 proteins are localized in both the cytoplasm and the nucleus, while BRUNOL4 and 5 are only present in the cytoplasm, indicating their different functions.

???displayArticle.pubmedLink??? 19757395
???displayArticle.link??? Int J Dev Biol

Species referenced: Xenopus laevis
Genes referenced: celf1 celf2 celf3 celf4 celf5

???attribute.lit??? ???displayArticles.show???

	Fig. 3. Embryonic expression of the X. laevis Brunol genes at the indicated stages. (A) Brunol1, (a,c,g), frontal views, dorsal to the top; (b,f,j,n), dorsal views, anterior to the left; (e,i,l,m), lateral views, anterior to the left.
	Fig. 3. Embryonic expression of the X. laevis Brunol genes at the indicated stages. (B) Brunol2, (a,b), lateral views, animal pole to the top; (c,d,h,l,p), dorsal views, anterior to the left; (f,i), frontal views, dorsal to the top; (g,k,n,o), lateral views, anterior to the left.
	Fig. 3. Embryonic expression of the X. laevis Brunol genes at the indicated stages.(C) Brunol3, (a,c,e,f), lateral views, anterior to the left; (b,g), dorsal views, anterior to the left.
	Fig. 3. Embryonic expression of the X. laevis Brunol genes at the indicated stages. (D) Brunol4 [celf4], (a), lateral view, anterior to the left; (b), dorsal view, anterior to the left. (E) Brunol5 [celf5], (a,b), lateral view, anterior to the left. The positions of the sections were indicate
	Fig. 4. Subcellular localization of the indicated Xenopus BRUNOL proteins. BRUNOL proteins were detected using anti-FLAG antibody. The cells were also stained with DAPI to visualize the nuclei.