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Dev Dyn 2006 Jul 01;2357:1895-907. doi: 10.1016/j.ydbio.2006.01.022.
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Novel gene ashwin functions in Xenopus cell survival and anteroposterior patterning.

Patil SS , Alexander TB , Uzman JA , Lou CH , Gohil H , Sater AK .

The novel gene ashwin was isolated in a differential display screen for genes activated or up-regulated early in neural specification. ashwin is expressed maternally and zygotically, and it is up-regulated in the neural ectoderm after the midgastrula stage. It is expressed in the neural plate and later in the embryonic brain, eyes, and spinal cord. Overexpression of ashwin in whole embryos leads to anterior truncations and other defects. However, a second Organizer does not form, and the secondary axial structures may result from splitting of the Organizer, rather than axis duplication. Morpholino oligonucleotide-mediated reduction in ashwin expression leads to lethality or abnormalities in gastrulation, as well as significant apoptosis in midgastrula embryos. Apoptosis is also observed in midgastrula embryos overexpressing ashwin. Coexpression of ashwin with the bone morphogenetic protein-4 antagonist noggin has a synergistic effect on neural-specific gene expression in isolated animal cap ectoderm. Ashwin has no previously characterized domains, although two nuclear localization signals can be identified. Orthologues have been identified in the human, mouse, chicken, and pufferfish genomes. Our results suggest that ashwin regulates cell survival and anteroposterior patterning.

PubMed ID: 16680723
Article link: Dev Dyn

Species referenced: Xenopus laevis
Genes referenced: bmp4 c2h2orf49 eef1a1 egr2 en2 frmd6 gsc hoxb9 msx1 mtor myc ncam1 nog not otx2 tubb2b ventx1.2 ventx2.2 zic3
Antibodies: Notochord Ab1
Morpholinos: c2orf49 MO1 c2orf49 MO2

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