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Dev Biol 2006 Apr 15;2922:442-56. doi: 10.1016/j.ydbio.2006.01.022.
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The RNA-binding protein, Vg1RBP, is required for pancreatic fate specification.

Signaling mechanisms underlying the induction of the pre-pancreatic tissue within the endoderm remain poorly understood. Through an expression cloning strategy, we have identified a previously uncharacterized pancreatic factor that we named Shirin. Interestingly, the non-coding RNA regulatory sequence (3 UTR) of Shirin is sufficient to induce insulin expression in Xenopus embryos. Biochemical studies demonstrate that this RNA sequence is able to bind directly to a trans-acting factor, Vg1RBP, which was previously shown to be involved in the localization of endodermal determinant factors. Loss-of-function analysis indicates that Vg1RBP is required for establishment of pancreatic fate within the endoderm, suggesting a synergism between Vg1RBP and Shirin in the embryo. This study argues for a central role of post-transcriptional mechanisms in establishing pancreatic fate, where a 3 UTR may recruit factors necessary for pancreatic development, and highlights an unknown embryological activity of Vg1RBP.

PubMed ID: 16680827
Article link: Dev Biol

Species referenced: Xenopus
Genes referenced: arhgap1 arhgef7 fabp2 gata5 gdf1 hhex hnrnpc igf2bp3 ins mixer mmut myc odc1 pdx1 ptf1a sox17a stard13 tbxt vegt

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