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J Assoc Res Otolaryngol
2007 Jun 01;82:205-19. doi: 10.1007/s10162-007-0072-0.
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Identification of ClC-2 and CIC-K2 chloride channels in cultured rat type IV spiral ligament fibrocytes.
Qu C
,
Liang F
,
Smythe NM
,
Schulte BA
.
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Voltage-gated chloride channels (ClCs) are important mediators of cellular ion homeostasis and volume regulation. In an earlier study, we used immunohistochemical, Western blot, and reverse transcriptase PCR (RT-PCR) approaches to identify ClC-K variants in types II, IV, and V fibrocytes of the rodent spiral ligament. We have now confirmed the expression of ClC-K2 in these cells by in situ hybridization. All three of these fibrocyte subtypes are thought to be involved in cochlear K(+) recycling; thus, it is important to understand the precise mechanisms regulating their membrane conductance and the role played by ClCs in this process. In this study, we report the characterization of a secondary cell line derived from explants from the region of the rat spiral ligament underlying and inferior to the spiral prominence. The cultured cells were immunopositive for vimentin, Na,K/ATPase, Na,K,Cl-cotransporter, carbonic anhydrase isozyme II, and creatine kinase isozyme BB, but not for cytokeratins or Ca/ATPase, an immunostaining profile indicative of the type IV subtype. Evaluation of the cultures by RT-PCR and Western blot analysis confirmed the presence of both ClC-2 and -K2. Whole-cell patch clamp recordings identified two biophysically distinct Cl(-) currents in the cultured cells. One, an inwardly rectifying Cl(-) current activated by hyperpolarization or decreasing extracellular pH corresponded with the properties of ClC-2. The other, a weak outwardly rectifying Cl(-) current regulated by extracellular pH, Cl(-), and Ca(2+) resembled the channel characteristics of ClC-K2 when expressed in Xenopus oocytes. These findings suggest that at least two functionally different chloride channels are involved in regulating membrane anion conductance in cultured type IV spiral ligament fibrocytes.
Ando,
mRNA encoding 'ClC-K1, a kidney Cl(-)- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl(-) currents.
2000, Pubmed,
Xenbase
Ando,
mRNA encoding 'ClC-K1, a kidney Cl(-)- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: its possible contribution to Cl(-) currents.
2000,
Pubmed
,
Xenbase
Auzanneau,
A Novel voltage-dependent chloride current activated by extracellular acidic pH in cultured rat Sertoli cells.
2003,
Pubmed
,
Xenbase
Boettger,
Deafness and renal tubular acidosis in mice lacking the K-Cl co-transporter Kcc4.
2002,
Pubmed
Chen,
Structure and function of clc channels.
2005,
Pubmed
Clayton,
Changes in liver-specific compared to common gene transcription during primary culture of mouse hepatocytes.
1983,
Pubmed
Crouch,
Immunohistochemical localization of the Na-K-Cl co-transporter (NKCC1) in the gerbil inner ear.
1997,
Pubmed
Davies,
Chloride channel gene expression in the rabbit cornea.
2004,
Pubmed
Enz,
Expression of the voltage-gated chloride channel ClC-2 in rod bipolar cells of the rat retina.
1999,
Pubmed
Estévez,
Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.
2001,
Pubmed
,
Xenbase
Estévez,
CLC chloride channels: correlating structure with function.
2002,
Pubmed
GOLDBERG,
AN ANALYSIS OF COLLAGEN SECRETION BY ESTABLISHED MOUSE FIBROBLAST LINES.
1964,
Pubmed
Gratton,
Characterization and development of an inner ear type I fibrocyte cell culture.
1996,
Pubmed
Gründer,
Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.
,
Pubmed
,
Xenbase
Hedman,
External fibronectin of cultured human fibroblasts is predominantly a matrix protein.
1978,
Pubmed
Huber,
CFTR mRNA and its truncated splice variant (TRN-CFTR) are differentially expressed during collecting duct ontogeny.
1998,
Pubmed
Inoue,
Evolutionary conservation of the insulinoma gene rig and its possible function.
1987,
Pubmed
Jentsch,
The ClC family of voltage-gated chloride channels: structure and function.
1993,
Pubmed
,
Xenbase
Jentsch,
CLC chloride channels and transporters.
2005,
Pubmed
Jentsch,
Properties of voltage-gated chloride channels of the ClC gene family.
1995,
Pubmed
Jordt,
Molecular dissection of gating in the ClC-2 chloride channel.
1997,
Pubmed
,
Xenbase
Kawasaki,
Single-cell RT-PCR demonstrates expression of voltage-dependent chloride channels (ClC-1, ClC-2 and ClC-3) in outer hair cells of rat cochlea.
1999,
Pubmed
Kawasaki,
mRNA expression of kidney-specific ClC-K1 chloride channel in single-cell reverse transcription-polymerase chain reaction analysis of outer hair cells of rat cochlea.
2000,
Pubmed
,
Xenbase
Kulka,
Mast cells express chloride channels of the ClC family.
2002,
Pubmed
Kunzelmann,
The cystic fibrosis transmembrane conductance regulator and its function in epithelial transport.
1999,
Pubmed
Liang,
A voltage- and Ca2+-dependent big conductance K channel in cochlear spiral ligament fibrocytes.
2003,
Pubmed
Matsumura,
Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1 chloride channel.
1999,
Pubmed
Neveu,
Differences in the expression of connexin genes in rat hepatomas in vivo and in vitro.
1994,
Pubmed
Obermüller,
The swelling-activated chloride channel ClC-2, the chloride channel ClC-3, and ClC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells.
1998,
Pubmed
Olsen,
Expression of voltage-gated chloride channels in human glioma cells.
2003,
Pubmed
Oshima,
Expression of voltage-dependent chloride channels in the rat cochlea.
1997,
Pubmed
Qu,
Expression of CLC-K chloride channels in the rat cochlea.
2006,
Pubmed
Riordan,
Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.
1989,
Pubmed
Sage,
Immunolocalization of ClC-K chloride channel in strial marginal cells and vestibular dark cells.
2001,
Pubmed
Schlingmann,
Salt wasting and deafness resulting from mutations in two chloride channels.
2004,
Pubmed
,
Xenbase
Schwiebert,
Analysis of ClC-2 channels as an alternative pathway for chloride conduction in cystic fibrosis airway cells.
1998,
Pubmed
Sharabani-Yosef,
Rat skeletal muscle in culture expresses the alpha1 but not the alpha2 protein subunit isoform of the Na+/K+ pump.
1999,
Pubmed
Shen,
BK channels mediate the voltage-dependent outward current in type I spiral ligament fibrocytes.
2004,
Pubmed
Simon,
Mutations in the chloride channel gene, CLCNKB, cause Bartter's syndrome type III.
1997,
Pubmed
Spicer,
Evidence for a medial K+ recycling pathway from inner hair cells.
1998,
Pubmed
Spicer,
Differentiation of inner ear fibrocytes according to their ion transport related activity.
1991,
Pubmed
Spicer,
The fine structure of spiral ligament cells relates to ion return to the stria and varies with place-frequency.
1996,
Pubmed
Steinmeyer,
Inactivation of muscle chloride channel by transposon insertion in myotonic mice.
1991,
Pubmed
Suko,
Classification and culture of spiral ligament fibrocytes from mice.
2000,
Pubmed
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
Vandewalle,
Localization and induction by dehydration of ClC-K chloride channels in the rat kidney.
1997,
Pubmed
Waldegger,
Functional and structural analysis of ClC-K chloride channels involved in renal disease.
2000,
Pubmed
,
Xenbase
Waldegger,
Barttin increases surface expression and changes current properties of ClC-K channels.
2002,
Pubmed
,
Xenbase
Yoshikawa,
Localization of rat CLC-K2 chloride channel mRNA in the kidney.
1999,
Pubmed
