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Dev Cell 2007 Jan 01;121:31-43. doi: 10.1016/j.devcel.2006.11.001.
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Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly.

Kelly AE , Sampath SC , Maniar TA , Woo EM , Chait BT , Funabiki H .

Chromatin-induced spindle assembly depends on regulation of microtubule-depolymerizing proteins by the chromosomal passenger complex (CPC), consisting of Incenp, Survivin, Dasra (Borealin), and the kinase Aurora B, but the mechanism and significance of the spatial regulation of Aurora B activity remain unclear. Here, we show that the Aurora B pathway is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activated by chromatin via a Ran-independent mechanism. While spindle microtubule assembly normally requires Dasra-dependent chromatin binding of the CPC, this function of Dasra can be bypassed by clustering Aurora B-Incenp by using anti-Incenp antibodies, which stimulate autoactivation among bound complexes. However, such chromatin-independent Aurora B pathway activation promotes centrosomal microtubule assembly and produces aberrant achromosomal spindle-like structures. We propose that chromosomal enrichment of the CPC results in local kinase autoactivation, a mechanism that contributes to the spatial regulation of spindle assembly and possibly to other mitotic processes.

PubMed ID: 17199039
PMC ID: PMC1892535
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus
Genes referenced: aurkb birc5 incenp ran
Antibodies: Tuba4b Ab2

References [+] :
Andersen, Mitotic chromatin regulates phosphorylation of Stathmin/Op18. 1997, Pubmed, Xenbase