Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Biol 2004 Jul 01;2711:75-86. doi: 10.1016/j.ydbio.2004.02.029.
Show Gene links Show Anatomy links

The Meis3 protein and retinoid signaling interact to pattern the Xenopus hindbrain.

Dibner C , Elias S , Ofir R , Souopgui J , Kolm PJ , Sive H , Pieler T , Frank D .

In Xenopus embryos, proper hindbrain formation requires activities of both XMeis3 protein and retinoic acid (RA) signaling. In this study, we show that XMeis3 protein and RA signaling differentially interact to regulate hindbrain patterning. The knockdown of XMeis3 protein prevented RA-caudalizing activity from inducing hindbrain marker expression in both explants and embryos. In contrast, inhibition of RA signaling differentially modulated XMeis3 activity. Target genes that are jointly activated by either RA or XMeis3 activities could not be efficiently induced by XMeis3 when RA signaling was inhibited. However, transcription of an XMeis3 target gene that is not an RA target gene was hyper-induced in the absence of retinoid signaling. Target genes jointly induced by RA or XMeis3 protein were synergistically activated in the presence of both activities, while RA treatment inhibits the ability of XMeis3 to activate transcription of neural genes that are not RA targets. HoxD1, an RA direct-target gene was also identified as an XMeis3 direct-target gene. HoxD1 protein acts downstream of XMeis3 to induce hindbrain marker gene transcription. To pattern the hindbrain, RA requires functional XMeis3 protein activity. XMeis3 protein appears crucial for initial hindbrain induction, whereas RA signaling defines the spatial limits of hindbrain gene expression by modifying XMeis3 protein activity.

PubMed ID: 15196951
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: adm aldh1a2 cyp26a1 eef1a2 egr2 eng gal.2 hoxa1 hoxb1 hoxb3 hoxb4 hoxb9 hoxc9-like hoxd1 meis3 pbx1
Morpholinos: meis3 MO1

Article Images: [+] show captions