Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biosynthesis, processing, and control of release of melanotropic peptides in the neurointermediate lobe of Xenopus laevis.
Loh YP
,
Gainer H
.
???displayArticle.abstract???
The neurointermediate lobes of dark-adapted toads Xenopus laevis were incubated for 30 min in [3H]arginine and then "chased" for various time periods. By use of this pulse-chase paradigm there were detected 10 trichloroacetic acid (TCA)-precipitable peptides separated on acid-urea polyacrylamide gels and one TCA-soluble peptide separated by high-voltage electrophoresis (pH 4.9) with melanotropic activity. Each of these peptides had a different degree of melanocyte stimulating hormone (MSH) activity as revealed by the Anolis skin bioassay. Three of these TCA-precipitable peptides comigrated with ACTH, beta-lipotrophin, and alpha-MSH on acid-urea gels. Evidence suggesting a precursor-product mode of biosynthesis of the melanotropic peptides is presented. 7 of the 10 TCA-precipitable peptides and the one TCA-soluble peptide with melanotropic activity were released into the medium. The half-time of release of the TCA-precipitable peptides was about 2 h, whereas the half-time of TCA-soluble peptide release was about 30 min. The release of these peptides was inhibited by 5 X 10(-5) M dopamine. Dopamine inhibition of release did not appear to affect the biosynthesis of the melanotropic peptides, but did appear to enhance the degradation of the newly synthesized TCA-soluble peptide in the tissue. White adaptation of the toads greatly decreased the biosynthesis of all of the TCA-precipitable melanotropic peptides.
BURGERS,
Melanophore-stimulating hormones in vertebrates.
1963, Pubmed
BURGERS,
Melanophore-stimulating hormones in vertebrates.
1963,
Pubmed
Baker,
Ability of various factors to oppose the stimulatory effect of dibutyrylcyclic AMP on the release of melanocyte-stimulating hormone by the rat pituitary in vitro.
1976,
Pubmed
Baker,
The separation of different forms of melanocyte-stimulating hormone from the rat neurointermediate lobe by polyacrylamide gel electrophoresis, with a note on rat neurophysins.
1973,
Pubmed
Bertagna,
[In vitro biosynthesis of bovine beta-lipotropic hormone].
1974,
Pubmed
Bower,
Biogenic amines and control of melanophore stimulating hormone release.
1974,
Pubmed
Bradbury,
Lipotropin: precursor to two biologically active peptides.
1976,
Pubmed
Celis,
Release of pituitary melanocyte-stimulating hormone by the oxytocin fragment, H-Cys-Tyr-Ile-Gln-Asn-Oh.
1971,
Pubmed
DAWSON,
Evidence for the termination of neurosecretory fibers within the pars intermedia of the hypophysis of the frog, Rana pipiens.
1953,
Pubmed
ETKIN,
Neurosecretory control of the pars intermedia.
1962,
Pubmed
Estivariz,
"Big" and "small" ACTH in the partes distalis and intermedia of rats and toads.
1975,
Pubmed
FAZAKERLEY,
SEPARATION OF AMINO ACIDS, AS COPPER CHELATES, FROM AMINO ACID, PROTEIN AND PEPTIDE MIXTURES.
1965,
Pubmed
Gainer,
Micro disc electrophoresis in sodium dodecyl sulfate: an application to the study of protein synthesis in individual, identified neurons.
1971,
Pubmed
Hadley,
Regulation of melanophore stimulating hormone (MSH) release.
1973,
Pubmed
Hadley,
Cellular mechanisms controlling melanophore stimulating hormone (MSH) release.
1975,
Pubmed
Hopkins,
The biosynthesis, intracellular transport, and packaging of melanocyte-stimulating peptides in the amphibian pars intermedia.
1972,
Pubmed
,
Xenbase
Hopkins,
Localization of adrenergic fibers in the amphibian pars intermedia by electron microscope autoradiography and their selective removal by 6-hydroxydopamine.
1971,
Pubmed
Jones,
Mouse pituitary tumor mRNA directed cell-free synthesis of polypeptides that are cross-reactive with adrenocorticotropic hormone antiserum.
1977,
Pubmed
KRIVOY,
On a possible role of beta-melanocyte stimulating hormone (beta-MSH) in the central nervous system of the Mammalia: an effect of beta-MSH in the spinal cord of the cat.
1961,
Pubmed
Kastin,
MSH activity in pituitaries of rats treated with hypothalamic extracts.
1966,
Pubmed
Loh,
Low molecular weight specific proteins in identified molluscan neurons. I. Synthesis and storage.
1975,
Pubmed
Lowry,
The evolution of vertebrate corticotrophin and melanocyte stimulating hormone.
1975,
Pubmed
Mains,
Biosynthesis of adrenocorticotropic hormone in mouse pituitary tumor cells.
1976,
Pubmed
Nakai,
Evidence for a doubly innervated secretory unit in the anuran pars intermedia. II. Electron microscopic studies.
1969,
Pubmed
Nakanishi,
A large product of cell-free translation of messenger RNA coding for corticotropin.
1976,
Pubmed
Olsson,
A relationship between adrenergic blockage and the secretory process in cells of the pars intermedia of the frog Rana pipiens.
1973,
Pubmed
Preslock,
Melanophore and adrenocortical stimulating activities of substances from the pars intermedia, pars distalis, hypothalamus and cerebral cortex of the frog, Rana pipiens.
1970,
Pubmed
Sandman,
Enhancement of attention in man with ACTH/MSH 4-10.
1975,
Pubmed
Scott,
Incorporation of 14C-labelled amino acids into corticotrophin-like intermediate lobe peptide and alpha-melanocyte-stimulating hormone by the rat pituitary neurointermediate lobe in vitro, and the identification of four new pars intermedia peptides.
1976,
Pubmed
Scott,
Pituitary peptide.
1973,
Pubmed
Silman,
Human foetal pituitary peptides and parturition.
1976,
Pubmed
Smith,
The effect of apomorphine and ergocriptine on the release of MSH by the pars intermedia of Rana pipiens.
1975,
Pubmed
TALEISNIK,
A MELANOCYTE-STIMULATING HORMONE-RELEASING FACTOR IN HYPOTHALAMIC EXTRACTS.
1965,
Pubmed
Taleisnik,
Melanocyte-stimulating hormone-releasing and inhibiting factors in two hypothalamic extracts.
1967,
Pubmed
Terlou,
Microspectrofluorometric identification of formaldehyde induced fluorescence in hypothalamic nuclei of Xenopus laevis tadpoles.
1974,
Pubmed
,
Xenbase
Terlou,
The development of a hypothalamic monoaminergic system for the regulation of the Pars intermedia activity in Xenopus laevis.
1973,
Pubmed
,
Xenbase
Thornton,
The effect of catecholamines and background color on the in vitro incorporation of labeled amino acids into the neurointermediate lobe of the pituitary in Xenopus laevis.
1974,
Pubmed
,
Xenbase
Tilders,
Re-introduction and evaluation of an accurate, high capacity bioassay for melanocyte-stimulating hormone using the skin of Anolis carolinensis in vitro.
1975,
Pubmed
Volcanes,
Stereological analysis of the effects of 6-hydroxydopamine on the ultrastructure of the melanocyte-stimulating hormone cell of the pars intermedia of the pituitary of Xenopus laevis.
1976,
Pubmed
,
Xenbase
Yalow,
Size heterogeneity of immunoreactive human ACTH in plasma and in extracts of pituitary glands and ACTH-producing thymoma.
1971,
Pubmed