XB-ART-2762
Gene Expr Patterns
2004 Nov 01;51:129-34. doi: 10.1016/j.modgep.2004.05.003.
Show Gene links
Show Anatomy links
Spatial and temporal expression patterns of Xenopus Nkx-2.3 gene in skin epidermis during metamorphosis.
???displayArticle.abstract???
Using PCR cloning, the mRNA of XNkx-2.3 gene, a Xenopus tinman homologue, was identified in a cDNA library prepared from thyroid hormone (T(3))-treated tadpole skin. Quantitative RT-PCR and RNase Protection Assay confirmed the expression of XNkx-2.3 in adult frog skin and its amount was similar to the amount found in heart. In situ hybridization indicated that XNkx-2.3 was expressed in the frog epidermis. Further analysis of XNkx-2.3 expression patterns demonstrates that it shares great similarities with a 63 kDa keratin, a well-characterized marker for skin maturation, in the following aspects. First, XNkx-2.3 was expressed in tadpole skin during metamorphosis (stages 55-59), but not in pre-metamorphic (stage 54) skin. Secondly, XNkx-2.3 expression in skin responded to T(3) stimulation because it could be precociously induced by T(3) at pre-metamorphic stage, both in tadpoles and in cultures of skin explants. Finally, the T(3)-induced appearance of XNkx-2.3 in head skin occurred earlier and at higher level than that in tail skin. These data suggest that XNkx-2.3 may be an important factor for skin maturation and may also serve as a good marker to indicate the maturation of Xenopus epidermis.
???displayArticle.pubmedLink??? 15533828
???displayArticle.link??? Gene Expr Patterns
Species referenced: Xenopus
Genes referenced: krt78.6 nkx2-3 nkx2-5
???attribute.lit??? ???displayArticles.show???