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J Cell Biol 1989 Sep 01;1093:983-9.
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Yeast proteins that recognize nuclear localization sequences.

Silver P , Sadler I , Osborne MA .

A variety of peptides can mediate the localization of proteins to the nucleus. We have identified yeast proteins of 70 and 59 kD that bind to nuclear localization peptides of SV-40 T antigen, Xenopus nucleoplasmin, and the yeast proteins Ga14 and histone H2B. These proteins are assayed by the binding of peptide-albumin conjugates to proteins immobilized on nitrocellulose filters. These binding proteins fractionate with nuclei and are extractable with salt but not detergent. Radiolabeled peptide-albumin conjugates also bind to isolated nuclei; the binding is saturable and can be extracted with salt. Different nuclear localization peptides compete with each other, implying that a single class of proteins is responsible for their recognition. The 70- and 59-kD proteins have the properties expected for a receptor that would act to direct proteins to the nucleus.

PubMed ID: 2670959
PMC ID: PMC2115749

Grant support: [+]

Species referenced: Xenopus
Genes referenced: h2bc21 npm1

References [+] :
Adam, Identification of specific binding proteins for a nuclear location sequence. 1989, Pubmed