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XB-ART-20566
J Mol Biol 1994 Nov 11;2435:840-5. doi: 10.1006/jmbi.1994.1686.
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Yeast TBP can replace its human homologue in the RNA polymerase I-specific multisubunit factor SL1.

Rudloff U , Stunnenberg HG , Keaveney M , Grummt I .


Abstract
Basic mechanisms of transcription initiation are conserved from yeast to man. However, in contrast to genes transcribed by RNA polymerases II and III, ribosomal gene transcription by RNA polymerase I (Pol I) is species-specific. Promoter selectivity is mediated by SL1/TIF-IB, a multiprotein complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs) which bind to DNA and nucleate the assembly of initiation complexes. Using a human cell line that expresses epitope-tagged yeast TBP, we have isolated a chimeric complex consisting of yeast TBP and human TAFs which faithfully promotes human rDNA transcription in vitro. This result argues that specific interactions between TBP and Pol I-specific TAFs have been evolutionarily conserved between distant species. In addition, this finding also underscores the importance of TAFs in determining promoter selectivity of Pol I.

PubMed ID: 7966304
Article link: J Mol Biol


Species referenced: Xenopus
Genes referenced: mef2d tbp