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J Cell Biol 2005 May 09;1693:399-404. doi: 10.1083/jcb.200501121.
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Molecular constituents of neuronal AMPA receptors.

Fukata Y , Tzingounis AV , Trinidad JC , Fukata M , Burlingame AL , Nicoll RA , Bredt DS .

Dynamic regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.

PubMed ID: 15883194
PMC ID: PMC2171936
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: apcs cacng2 crebbp dlg1 dlg4 gria1 gria2 grip1 hspa5 nsf osbpl8 pick1 psd

Article Images: [+] show captions
References [+] :
Chen, Impaired cerebellar synapse maturation in waggler, a mutant mouse with a disrupted neuronal calcium channel gamma subunit. 1999, Pubmed