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Biochim Biophys Acta 2006 May 01;17595:216-24. doi: 10.1016/j.bbaexp.2006.05.004.
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A novel Xenopus laevis larval keratin gene, xlk2: its gene structure and expression during regeneration and metamorphosis of limb and tail.

Tazawa I , Shimizu-Nishikawa K , Yoshizato K .

A novel cytokeratin (CK) gene, xlk2, was cloned from a cDNA library prepared from regenerating limbs of Xenopus larvae. The deduced amino acid sequence indicated that its product, XLK2, is a 48 kDa type I (acidic) CK and has a high similarity to CK13, 15, and 19 with the highest homology (58%) to mouse CK15. The gene of xlk2 exclusively expressed in basal cells of the bi-layered larval epidermis, but not in other cells in larvae and not in other periods of life. Its expression was down-regulated during spontaneous and thyroid hormone-induced metamorphosis. The basal cells of the apical epidermal cap (AEC) formed on the regenerate of larval limbs terminated the expression of xlk2, whereas those of the adjacent normal epidermis continued to express it. The AEC-basal cells did not re-express the gene in the regenerate. In contrast, the basal cells of the tail regenerate also once terminated the expression of xlk2, but was able to re-express xlk2 later, supporting a notion that the "de-differentiated" basal cells of the tail epidermal regenerate re-differentiate into larval normal epidermal cells.

PubMed ID: 16822559
Article link: Biochim Biophys Acta

Species referenced: Xenopus laevis
Genes referenced: eef1a1 krt12.4 krt12.5 krt15.1 krt15.2 krt62 slc4a1
GO keywords: metamorphosis [+]
Antibodies: Cytokeratin Ab3

Article Images: [+] show captions