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EMBO J
1997 Dec 15;1624:7372-81. doi: 10.1093/emboj/16.24.7372.
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Reaper-induced apoptosis in a vertebrate system.
Evans EK
,
Kuwana T
,
Strum SL
,
Smith JJ
,
Newmeyer DD
,
Kornbluth S
.
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The reaper protein of Drosophila melanogaster has been shown to be a central regulator of apoptosis in that organism. However, it has not been shown to function in any vertebrate nor have the cellular components required for its action been defined. In this report we show that reaper can induce rapid apoptosis in vitro using an apoptotic reconstitution system derived from Xenopus eggs. Moreover, we show that a subcellular fraction enriched in mitochondria is required for this process and that reaper, acting in conjunction with cytosolic factors, can trigger mitochondrial cytochrome c release. Bcl-2 antagonizes these effects, but high levels of reaper can overcome the Bcl-2 block. These results demonstrate that reaper can function in a vertebrate context, suggesting that reaper-responsive factors are conserved elements of the apoptotic program.
Bellamy,
Cell death in health and disease: the biology and regulation of apoptosis.
1995, Pubmed
Bellamy,
Cell death in health and disease: the biology and regulation of apoptosis.
1995,
Pubmed
Boldin,
Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
1996,
Pubmed
Bump,
Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35.
1995,
Pubmed
Chen,
Apoptotic activity of REAPER is distinct from signaling by the tumor necrosis factor receptor 1 death domain.
1996,
Pubmed
Chinnaiyan,
Interaction of CED-4 with CED-3 and CED-9: a molecular framework for cell death.
1997,
Pubmed
Chinnaiyan,
FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.
1995,
Pubmed
Chinnaiyan,
The cell-death machine.
1996,
Pubmed
Duan,
ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.
1996,
Pubmed
Duan,
RAIDD is a new 'death' adaptor molecule.
1997,
Pubmed
Evans,
Crk is required for apoptosis in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Farschon,
Temporal phases in apoptosis defined by the actions of Src homology 2 domains, ceramide, Bcl-2, interleukin-1beta converting enzyme family proteases, and a dense membrane fraction.
1997,
Pubmed
,
Xenbase
Golstein,
Fas bridging cell death and cytotoxicity: the reaper connection.
1995,
Pubmed
Hsu,
The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation.
1995,
Pubmed
Kluck,
The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis.
1997,
Pubmed
,
Xenbase
Kluck,
Cytochrome c activation of CPP32-like proteolysis plays a critical role in a Xenopus cell-free apoptosis system.
1997,
Pubmed
,
Xenbase
Lazebnik,
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.
1994,
Pubmed
Liu,
Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c.
1996,
Pubmed
Liu,
DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
1997,
Pubmed
Muzio,
FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.
1996,
Pubmed
Newmeyer,
Cell-free apoptosis in Xenopus egg extracts: inhibition by Bcl-2 and requirement for an organelle fraction enriched in mitochondria.
1994,
Pubmed
,
Xenbase
Nicholson,
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis.
1995,
Pubmed
Nuñez,
The Bcl-2 family of proteins: regulators of cell death and survival.
1994,
Pubmed
Pronk,
Requirement of an ICE-like protease for induction of apoptosis and ceramide generation by REAPER.
1996,
Pubmed
Reed,
Bcl-2 and the regulation of programmed cell death.
1994,
Pubmed
Spector,
Interaction between the C. elegans cell-death regulators CED-9 and CED-4.
1997,
Pubmed
Tewari,
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.
1995,
Pubmed
Vucic,
Characterization of reaper- and FADD-induced apoptosis in a lepidopteran cell line.
1997,
Pubmed
White,
Cell killing by the Drosophila gene reaper.
1996,
Pubmed
White,
Genetic control of programmed cell death in Drosophila.
1994,
Pubmed
Xue,
Inhibition of the Caenorhabditis elegans cell-death protease CED-3 by a CED-3 cleavage site in baculovirus p35 protein.
1995,
Pubmed
Yang,
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.
1997,
Pubmed
Yuan,
Molecular control of life and death.
1995,
Pubmed
