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Dev Cell 2006 Jul 01;111:69-79. doi: 10.1016/j.devcel.2006.04.019.
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XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation.

Hyodo-Miura J , Yamamoto TS , Hyodo AC , Iemura S , Kusakabe M , Nishida E , Natsume T , Ueno N .

To dissect the molecular mechanisms underlying convergent extension (CE), a prominent set of cell movements during Xenopus gastrulation, we performed a functional expression screen and identified a GTPase-activating protein for ADP ribosylation factors (ArfGAP), which we termed XGAP. We demonstrated that XGAP is required to confine or restrict the cellular protrusive activity to the mediolateral ends of cells, where XGAP is normally localized, and therefore for the proper intercalation of cells participating in CE. We also demonstrated that a C-terminal conserved domain of XGAP, but not its GAP activity, is required and sufficient for this intracellular localization and function. We further showed that XGAP physically interacts with the known polarity proteins 14-3-3epsilon, aPKC, and PAR-6 and directs them to the mediolateral ends of dorsal mesoderm cells during gastrulation. We propose that XGAP controls CE through the restriction and maintenance of partitioning-defective (PAR) proteins in the regions that harbor protrusive activity.

PubMed ID: 16824954
Article link: Dev Cell

Species referenced: Xenopus
Genes referenced: arf1 arf4 arf6 arf6.2 arfgap3 arg2 chrd ctrl fzd7 gga3 gsc myc myf5 not odc1 otx2 pard6b prkci tbx2 tbxt wnt11 wnt11b ywhae
Antibodies: Notochord Ab2 Somite Ab1
Morpholinos: arfgap3 MO1

Article Images: [+] show captions
References :
Mlodzik, A GAP in convergent extension scores PAR. 2006, Pubmed, Xenbase