XB-ART-10093Mech Dev 2000 Nov 01;981-2:99-104. doi: 10.1016/s0925-4773(00)00436-6.
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Distinct expression of two types of Xenopus Patched genes during early embryogenesis and hindlimb development.
Patched (Ptc) is a putative twelve transmembrane domain protein that is both a Hedgehog (Hh) receptor and transcriptional target of Hh. In this study, we isolated Xenopus Ptc cDNAs, Ptc-1 and Ptc-2, and carried out comparative analyses on their expression patterns. The putative Ptc-2 protein has a long C-terminal extension that has similarities in both length and sequence to those of Ptc-1 proteins in mouse, chick and human. In both early embryogenesis and hindlimb development, Ptc-2 expression is restricted to cells that receive a Hh signal, a pattern similar to that of Gli-1. Ptc-1, however, shows a broader distribution, mainly non-overlapping with that of Ptc-2. Despite the difference in their expression patterns, both are induced in animal cap explants synergistically by Shh and Noggin, showing a conserved regulation in their activation mechanisms.
PubMed ID: 11044611
Article link: Mech Dev
Species referenced: Xenopus
Genes referenced: gli1 gli2 gli3 ihh nog ptch1 ptch2 shh
Article Images: [+] show captions
|Fig. 3. Whole-mount in situ hybridization showing the localization of Ptc-1 (A–C,S,V,X), Ptc-2 (D–F,T,W,Y), Shh (G–I,U,Z), Gli-1 (J–L), Gli-2 (M–O) and Gli-3 (P–R). (A,D,G,J,M,P) Dorsal views at stages 14. (B,E,H,K,N,Q) Lateral views at stage 26. (C,F,I,L,O,R) Lateral views at stage 31. (S–U) Lateral views of facial regions at stage 31. (V, W) Transverse sections at stage 14. (X–Z) Transverse sections through spinal cord and notochord at stage 27. Embryos in (B,C,E,F,H,I,K,L,N,O,Q–U) were cleared by benzyl alcohol-benzyl benzoate.|
|Fig. 4. Expression of Ptc, Gli-1 and Hh genes in developing hindlimbs. All are dorsal views. An inset in Xptc-1 (stage 52) is a transverse section of the limb bud oriented with the posterior and dorsal sides facing down and to the left, respectively.|
|shh (sonic hedgehog) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.|